Literature DB >> 24652621

High-throughput screening for novel anti-infectives using a C. elegans pathogenesis model.

Annie L Conery1,2, Jonah Larkins-Ford1, Frederick M Ausubel1,2, Natalia V Kirienko1,2.   

Abstract

In recent history, the nematode Caenorhabditis elegans has provided a compelling platform for the discovery of novel antimicrobial drugs. In this protocol, we present an automated, high-throughput C. elegans pathogenesis assay, which can be used to screen for anti-infective compounds that prevent nematodes from dying due to Pseudomonas aeruginosa. New antibiotics identified from such screens would be promising candidates for treatment of human infections, and also can be used as probe compounds to identify novel targets in microbial pathogenesis or host immunity.
Copyright © 2014 John Wiley & Sons, Inc.

Entities:  

Keywords:  Caenorhabditis elegans; Pseudomonas aeruginosa; antibiotic; antimicrobial; drug discovery; high-throughput screening; liquid killing; pathogenesis model

Mesh：

Substances：

Year:  2014        PMID： 24652621      PMCID： PMC4021578          DOI： 10.1002/9780470559277.ch130160

Source DB:  PubMed          Journal:  Curr Protoc Chem Biol        ISSN： 2160-4762


  29 in total

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Authors: 
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3.  Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis.

Authors:  M W Tan; S Mahajan-Miklos; F M Ausubel
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Authors:  Costi D Sifri; Jakob Begun; Frederick M Ausubel
Journal:  Trends Microbiol       Date:  2005-03       Impact factor: 17.079

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Authors:  A Labrousse; S Chauvet; C Couillault; C L Kurz; J J Ewbank
Journal:  Curr Biol       Date:  2000-11-30       Impact factor: 10.834

7.  A simple model host for identifying Gram-positive virulence factors.

Authors:  D A Garsin; C D Sifri; E Mylonakis; X Qin; K V Singh; B E Murray; S B Calderwood; F M Ausubel
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8.  High-throughput screen for novel antimicrobials using a whole animal infection model.

Authors:  Terence I Moy; Annie L Conery; Jonah Larkins-Ford; Gang Wu; Ralph Mazitschek; Gabriele Casadei; Kim Lewis; Anne E Carpenter; Frederick M Ausubel
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Review 3.  High-throughput screening in the C. elegans nervous system.

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7.  Algorithmic Mapping and Characterization of the Drug-Induced Phenotypic-Response Space of Parasites Causing Schistosomiasis.

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10.  Imaging and Fluorescence Quantification in Caenorhabditis eleganswith Flow Vermimetry and Automated Microscopy.

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