Literature DB >> 30204302

RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans.

Sakthimala Jagadeesan1,2, Abdul Hakkim1,2.   

Abstract

RNAi is a powerful reverse genetics tool that has revolutionized genetic studies in model organisms. The bacteriovorous nematode Caenorhabditis elegans can be genetically manipulated by feeding it an Escherichia coli strain that expresses a double-stranded RNA (dsRNA) corresponding to a C. elegans gene, which leads to systemic silencing of the gene. This unit describes protocols for performing an automated high-throughput RNAi screen utilizing a full-genome C. elegans RNAi library. The protocols employ liquid-handling robotics and 96-well plates.
© 2018 by John Wiley & Sons, Inc. © 2018 John Wiley & Sons, Inc.

Entities:  

Keywords:  C. elegans; RNAi; RNAi feeding; automated high-throughput screening; reverse genetics

Mesh：

Substances：

Year:  2018        PMID： 30204302      PMCID： PMC6168420          DOI： 10.1002/cpmb.65

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


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