Zachary S Peacock1, Brad J Tricomi2, Matthew E Lawler3, William C Faquin4, John C Magill5, Brian A Murphy6, Leonard B Kaban7, Maria J Troulis8. 1. Assistant Professor, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston, MA. Electronic address: zpeacock@partners.org. 2. Masters Student, University of Massachusetts Boston; Research Fellow, Massachusetts General Hospital, Boston, MA. 3. Current Resident, Former Synthes/Craniomaxillofacial Student Research Fellow, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston, MA. 4. Associate Professor, Department of Pathology, Massachusetts General Hospital, Boston, MA. 5. Principal Research Scientist, Physical Sciences, Inc, Andover, MA. 6. Principal Scientist, Physical Sciences, Inc, Andover, MA. 7. Walter C. Guralnick Professor and Chairman, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston, MA. 8. Associate Professor, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston, MA.
Abstract
PURPOSE: To document the bone formation and soft tissue changes in response to automated, continuous, curvilinear distraction osteogenesis (DO) at rates greater than 1 mm/day in a minipig model. MATERIALS AND METHODS: Two groups of Yucatan minipigs underwent automated, continuous, curvilinear DO of the right mandible: group A, 1.5 mm/day (n = 5); and group B, 3.0 mm/day (n = 5). Each minipig underwent 12 mm of distraction followed by 24 days of fixation. The distracted and contralateral mandibles were harvested at the end of fixation. The percentage of surface area (PSA) of the regenerate occupied by bone, fibrous tissue, cartilage, and hematoma was determined using computerized histomorphometric analysis. The control groups consisted of DO wounds distracted discontinuously at 1 mm/day and the nonoperated contralateral mandible. The ipsilateral and contralateral digastric muscles were harvested and stained for proliferating cell nuclear antigen (PCNA), myogenic differentiation-1 (MyoD), and paired Box 7 protein (PAX7). RESULTS: All 10 minipigs completed the distraction and fixation period. The PSA occupied by bone was similar for groups A (PSA 64.36% ± 5.87%) and B (PSA 63.83% ± 3.37%) and the control group (1 mm/day; PSA 64.89% ± 0.56%) but was less than that on the nonoperated side (PSA 84.67% ± 0.86%). The PSA occupied by cartilage and hematoma in all groups was minimal (<1.1%). The digastric muscles had no abnormal tissue or inflammation, and PAX7, MyoD, and PCNA expression had returned to the baseline levels. CONCLUSIONS: The results of the present study indicate that bone formation in response to automated, continuous, and curvilinear DO at a rate of 1.5 and 3.0 mm/day is nearly identical to that with discontinuous DO at 1 mm/day. In addition, no deleterious effects were found on the digastric muscles.
PURPOSE: To document the bone formation and soft tissue changes in response to automated, continuous, curvilinear distraction osteogenesis (DO) at rates greater than 1 mm/day in a minipig model. MATERIALS AND METHODS: Two groups of Yucatan minipigs underwent automated, continuous, curvilinear DO of the right mandible: group A, 1.5 mm/day (n = 5); and group B, 3.0 mm/day (n = 5). Each minipig underwent 12 mm of distraction followed by 24 days of fixation. The distracted and contralateral mandibles were harvested at the end of fixation. The percentage of surface area (PSA) of the regenerate occupied by bone, fibrous tissue, cartilage, and hematoma was determined using computerized histomorphometric analysis. The control groups consisted of DO wounds distracted discontinuously at 1 mm/day and the nonoperated contralateral mandible. The ipsilateral and contralateral digastric muscles were harvested and stained for proliferating cell nuclear antigen (PCNA), myogenic differentiation-1 (MyoD), and paired Box 7 protein (PAX7). RESULTS: All 10 minipigs completed the distraction and fixation period. The PSA occupied by bone was similar for groups A (PSA 64.36% ± 5.87%) and B (PSA 63.83% ± 3.37%) and the control group (1 mm/day; PSA 64.89% ± 0.56%) but was less than that on the nonoperated side (PSA 84.67% ± 0.86%). The PSA occupied by cartilage and hematoma in all groups was minimal (<1.1%). The digastric muscles had no abnormal tissue or inflammation, and PAX7, MyoD, and PCNA expression had returned to the baseline levels. CONCLUSIONS: The results of the present study indicate that bone formation in response to automated, continuous, and curvilinear DO at a rate of 1.5 and 3.0 mm/day is nearly identical to that with discontinuous DO at 1 mm/day. In addition, no deleterious effects were found on the digastric muscles.
Authors: Lutz Ritter; Krishna Yeshwant; Edward B Seldin; Leonard B Kaban; Jaime Gateno; Erwin Keeve; Ron Kikinis; Maria J Troulis Journal: J Oral Maxillofac Surg Date: 2006-02 Impact factor: 1.895
Authors: John C Magill; Marten F Byl; Batya Goldwaser; Maria Papadaki; Roger Kromann; Brent Yates; Joseph R Morency; Leonard B Kaban; Maria J Troulis Journal: J Med Device Date: 2009-03 Impact factor: 0.582
Authors: B J Mehrara; N M Rowe; D S Steinbrech; M E Dudziak; P B Saadeh; J G McCarthy; G K Gittes; M T Longaker Journal: Plast Reconstr Surg Date: 1999-02 Impact factor: 4.730
Authors: Zachary S Peacock; Brad J Tricomi; William C Faquin; John C Magill; Brian A Murphy; Leonard B Kaban; Maria J Troulis Journal: J Craniofac Surg Date: 2015-11 Impact factor: 1.046