Literature DB >> 24590776

Poly(A) polymerase-based poly(A) length assay.

Deepak P Patil1, Baskar Bakthavachalu, Daniel R Schoenberg.   

Abstract

mRNA polyadenylation functions in nuclear export, translation, and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.

Entities:  

Mesh：

Substances：

Year:  2014        PMID： 24590776      PMCID： PMC3951053          DOI： 10.1007/978-1-62703-971-0_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells.

Authors:  Elizabeth L Murray; Daniel R Schoenberg
Journal:  Methods Enzymol       Date:  2008       Impact factor: 1.600

2.  Identification of two cis-acting elements that independently regulate the length of poly(A) on Xenopus albumin pre-mRNA.

Authors:  J Das Gupta; H Gu; E Chernokalskaya; X Gao; D R Schoenberg
Journal:  RNA       Date:  1998-07       Impact factor: 4.942

3.  Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish.

Authors:  Ariel A Bazzini; Miler T Lee; Antonio J Giraldez
Journal:  Science       Date:  2012-03-15       Impact factor: 47.728

4.  A new G-tailing method for the determination of the poly(A) tail length applied to hepatitis A virus RNA.

Authors:  Y Y Kusov; G Shatirishvili; G Dzagurov; V Gauss-Müller
Journal:  Nucleic Acids Res       Date:  2001-06-15       Impact factor: 16.971

5.  Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.

Authors:  G Martin; W Keller
Journal:  RNA       Date:  1998-02       Impact factor: 4.942

6.  Characterization of the E.coli poly(A) polymerase: nucleotide specificity, RNA-binding affinities and RNA structure dependence.

Authors:  S Yehudai-Resheff; G Schuster
Journal:  Nucleic Acids Res       Date:  2000-03-01       Impact factor: 16.971

7.  Genomewide characterization of non-polyadenylated RNAs.

Authors:  Li Yang; Michael O Duff; Brenton R Graveley; Gordon G Carmichael; Ling-Ling Chen
Journal:  Genome Biol       Date:  2011-02-16       Impact factor: 13.583

Review 8.  Quadruplex DNA: sequence, topology and structure.

Authors:  Sarah Burge; Gary N Parkinson; Pascale Hazel; Alan K Todd; Stephen Neidle
Journal:  Nucleic Acids Res       Date:  2006-09-29       Impact factor: 16.971
  8 in total
  5 in total

1.  Exploring Translational Control of Maternal mRNAs in Zebrafish.

Authors:  Cecilia Lanny Winata; Maciej Łapiński; Hisyam Ismail; Sinnakaruppan Mathavan; Prabha Sampath
Journal:  Methods Mol Biol       Date:  2021

Review 2.  Determinants and implications of mRNA poly(A) tail size--does this protein make my tail look big?

Authors:  Aimee L Jalkanen; Stephen J Coleman; Jeffrey Wilusz
Journal:  Semin Cell Dev Biol       Date:  2014-06-05       Impact factor: 7.727

3.  Characterization of the Role of Hexamer AGUAAA and Poly(A) Tail in Coronavirus Polyadenylation.

Authors:  Yu-Hui Peng; Ching-Houng Lin; Chao-Nan Lin; Chen-Yu Lo; Tsung-Lin Tsai; Hung-Yi Wu
Journal:  PLoS One       Date:  2016-10-19       Impact factor: 3.240

4.  Phosphodiester modifications in mRNA poly(A) tail prevent deadenylation without compromising protein expression.

Authors:  Dominika Strzelecka; Miroslaw Smietanski; Pawel J Sikorski; Marcin Warminski; Joanna Kowalska; Jacek Jemielity
Journal:  RNA       Date:  2020-08-20       Impact factor: 4.942

5.  Newly synthesized mRNA escapes translational repression during the acute phase of the mammalian unfolded protein response.

Authors:  Mohammed R Alzahrani; Bo-Jhih Guan; Leah L Zagore; Jing Wu; Chien-Wen Chen; Donny D Licatalosi; Kristian E Baker; Maria Hatzoglou
Journal:  PLoS One       Date:  2022-08-10       Impact factor: 3.752
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.