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Literature DB >> 9570322

Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.

Abstract

We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.

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Year: 1998 PMID： 9570322 PMCID： PMC1369611

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

21 in total

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