Literature DB >> 2455893

Quantitative analysis of RNA produced by slow and fast alleles of Adh in Drosophila melanogaster.

C C Laurie1, L F Stam.   

Abstract

The alcohol dehydrogenase (ADH) locus (Adh) of Drosophila melanogaster in polymorphic on a world-wide basis for two allozymes, Fast and Slow. This study was undertaken to determine whether the well-established difference in ADH protein concentration between the allozymes is due to a difference in mRNA levels. RNA gel blot hybridization and an RNase protection assay were used to quantify ADH mRNA levels. Each method used an Adh null mutant as an internal standard. Several Slow and Fast allele pairs of different geographic origins were analyzed. The results provide strong evidence that the ADH protein concentration difference is not accounted for by RNA level.

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Year:  1988        PMID： 2455893      PMCID： PMC281708          DOI： 10.1073/pnas.85.14.5161

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  The alcohol dehydrogenase alleloenzymes AdhS and AdhF from the fruitfly Drosophila melanogaster: an enzymatic rate assay to determine the active-site concentration.

Authors:  J O Winberg; R Hovik; J S McKinley-McKee
Journal:  Biochem Genet       Date:  1985-04       Impact factor: 1.890

2.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

3.  Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila.

Authors:  C Benyajati; A R Place; N Wang; E Pentz; W Sofer
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

4.  The relative quantities and catalytic activities of enzymes produced by alleles at the alcohol dehydrogenase locus in Drosophila melanogaster.

Authors:  T H Day; P C Hillier; B Clarke
Journal:  Biochem Genet       Date:  1974-02       Impact factor: 1.890

5.  Genetic control of alcohol dehydrogenase levels in Drosophila.

Authors:  G Maroni
Journal:  Biochem Genet       Date:  1978-06       Impact factor: 1.890

6.  The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.

Authors:  C Benyajati; N Spoerel; H Haymerle; M Ashburner
Journal:  Cell       Date:  1983-05       Impact factor: 41.582

7.  Formaldehyde mutagenesis in Drosophila. Molecular analysis of ADH-negative mutants.

Authors:  C Benyajati; A R Place; W Sofer
Journal:  Mutat Res       Date:  1983-09       Impact factor: 2.433

8.  Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster.

Authors:  C C Laurie-Ahlberg; L F Stam
Journal:  Genetics       Date:  1987-01       Impact factor: 4.562

9.  Biochemical differences between products of the Adh locus in Drosophila.

Authors:  J F McDonald; S M Anderson; M Santos
Journal:  Genetics       Date:  1980-08       Impact factor: 4.562

10.  Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.

Authors:  D A Goldberg; J W Posakony; T Maniatis
Journal:  Cell       Date:  1983-08       Impact factor: 41.582
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  17 in total

Review 1.  Experimental approaches to evaluate the contributions of candidate protein-coding mutations to phenotypic evolution.

Authors:  Jay F Storz; Anthony J Zera
Journal:  Methods Mol Biol       Date:  2011

2.  Inferring the evolutionary histories of the Adh and Adh-dup loci in Drosophila melanogaster from patterns of polymorphism and divergence.

Authors:  M Kreitman; R R Hudson
Journal:  Genetics       Date:  1991-03       Impact factor: 4.562

3.  Analysis of sequences regulating larval expression of the Adh gene of Drosophila melanogaster.

Authors:  N L Shen; E C Hotaling; G Subrahmanyam; P F Martin; W Sofer
Journal:  Genetics       Date:  1991-11       Impact factor: 4.562

4.  Translation initiation in Drosophila melanogaster is reduced by mutations upstream of the AUG initiator codon.

Authors:  Y Feng; L E Gunter; E L Organ; D R Cavener
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

5.  RNA folding in Drosophila shows a distance effect for compensatory fitness interactions.

Authors:  W Stephan; D A Kirby
Journal:  Genetics       Date:  1993-09       Impact factor: 4.562

Review 6.  Evolutionary genetics of the Drosophila alcohol dehydrogenase gene-enzyme system.

Authors:  P W Heinstra
Journal:  Genetica       Date:  1993       Impact factor: 1.082

7.  Sequence variation of alcohol dehydrogenase (Adh) paralogs in cactophilic Drosophila.

Authors:  Luciano M Matzkin; Walter F Eanes
Journal:  Genetics       Date:  2003-01       Impact factor: 4.562

8.  Molecular dissection of a major gene effect on a quantitative trait: the level of alcohol dehydrogenase expression in Drosophila melanogaster.

Authors:  L F Stam; C C Laurie
Journal:  Genetics       Date:  1996-12       Impact factor: 4.562

9.  The effect of an intronic polymorphism on alcohol dehydrogenase expression in Drosophila melanogaster.

Authors:  C C Laurie; L F Stam
Journal:  Genetics       Date:  1994-10       Impact factor: 4.562

10.  A highly conserved sequence in the 3'-untranslated region of the drosophila Adh gene plays a functional role in Adh expression.

Authors:  J Parsch; W Stephan; S Tanda
Journal:  Genetics       Date:  1999-02       Impact factor: 4.562
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