Literature DB >> 24523474

Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system.

Marjeta Hofko1, Alexander Mischnik, Martin Kaase, Stefan Zimmermann, Alexander H Dalpke.   

Abstract

We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

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Year:  2014        PMID： 24523474      PMCID： PMC3993711          DOI： 10.1128/JCM.00373-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Rapid detection of carbapenemase genes by multiplex real-time PCR.

Authors:  Jussimara Monteiro; Raymond H Widen; Antonio C C Pignatari; Carly Kubasek; Suzane Silbert
Journal:  J Antimicrob Chemother       Date:  2012-01-09       Impact factor: 5.790

Review 2.  Identification and screening of carbapenemase-producing Enterobacteriaceae.

Authors:  P Nordmann; M Gniadkowski; C G Giske; L Poirel; N Woodford; V Miriagou
Journal:  Clin Microbiol Infect       Date:  2012-05       Impact factor: 8.067

3.  Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae.

Authors:  Sandrine Bernabeu; Laurent Poirel; Patrice Nordmann
Journal:  Diagn Microbiol Infect Dis       Date:  2012-06-22       Impact factor: 2.803

4.  Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.

Authors:  Martin Kaase; Florian Szabados; Lars Wassill; Sören G Gatermann
Journal:  J Clin Microbiol       Date:  2012-07-11       Impact factor: 5.948

5.  Sensitive EDTA-based microbiological assays for detection of metallo-{beta}-lactamases in nonfermentative gram-negative bacteria.

Authors:  Patricia Marchiaro; María A Mussi; Viviana Ballerini; Fernando Pasteran; Alejandro M Viale; Alejandro J Vila; Adriana S Limansky
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

Review 6.  Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues.

Authors:  V Miriagou; G Cornaglia; M Edelstein; I Galani; C G Giske; M Gniadkowski; E Malamou-Lada; L Martinez-Martinez; F Navarro; P Nordmann; L Peixe; S Pournaras; G M Rossolini; A Tsakris; A Vatopoulos; R Cantón
Journal:  Clin Microbiol Infect       Date:  2010-02       Impact factor: 8.067

7.  Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours.

Authors:  Irene Burckhardt; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2011-07-27       Impact factor: 5.948

8.  A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin.

Authors:  C G Giske; L Gezelius; Ø Samuelsen; M Warner; A Sundsfjord; N Woodford
Journal:  Clin Microbiol Infect       Date:  2011-04       Impact factor: 8.067

9.  Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae.

Authors:  Fernando Pasteran; Tania Mendez; Leonor Guerriero; Melina Rapoport; Alejandra Corso
Journal:  J Clin Microbiol       Date:  2009-04-22       Impact factor: 5.948

10.  Rapid detection of carbapenemase-producing Enterobacteriaceae.

Authors:  Patrice Nordmann; Laurent Poirel; Laurent Dortet
Journal:  Emerg Infect Dis       Date:  2012-09       Impact factor: 6.883
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  11 in total

1.  Development of a Real-Time PCR Protocol Requiring Minimal Handling for Detection of Vancomycin-Resistant Enterococci with the Fully Automated BD Max System.

Authors:  Alexander H Dalpke; Marjeta Hofko; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2016-06-29       Impact factor: 5.948

2.  Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers.

Authors:  Laurent Poirel; Patrice Nordmann
Journal:  J Clin Microbiol       Date:  2015-06-17       Impact factor: 5.948

3.  Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples.

Authors:  Tracy D Lee; Kathleen Adie; Alan McNabb; Dale Purych; Kulvinder Mannan; Robert Azana; Corrinne Ng; Patrick Tang; Linda M N Hoang
Journal:  J Clin Microbiol       Date:  2015-05-27       Impact factor: 5.948

4.  Development and optimization of a real-time PCR assay for detection of herpes simplex and varicella-zoster viruses in skin and mucosal lesions by use of the BD Max open system.

Authors:  Ana María Cárdenas; Paul H Edelstein; Kevin Alby
Journal:  J Clin Microbiol       Date:  2014-10-01       Impact factor: 5.948

Review 5.  The Global Ascendency of OXA-48-Type Carbapenemases.

Authors:  Johann D D Pitout; Gisele Peirano; Marleen M Kock; Kathy-Anne Strydom; Yasufumi Matsumura
Journal:  Clin Microbiol Rev       Date:  2019-11-13       Impact factor: 26.132

6.  Rapid detection of four non-fermenting Gram-negative bacteria directly from cystic fibrosis patient's respiratory samples on the BD MAX™ system.

Authors:  Talita T Rocchetti; Suzane Silbert; Alicia Gostnell; Carly Kubasek; Robert Jerris; Jennifer Vong; Raymond Widen
Journal:  Pract Lab Med       Date:  2018-05-24

7.  Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.

Authors:  Talita T Rocchetti; Suzane Silbert; Alicia Gostnell; Carly Kubasek; Raymond Widen
Journal:  J Clin Microbiol       Date:  2016-03-23       Impact factor: 5.948

8.  Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates.

Authors:  M Smiljanic; M Kaase; P Ahmad-Nejad; B Ghebremedhin
Journal:  Ann Clin Microbiol Antimicrob       Date:  2017-07-10       Impact factor: 3.944

9.  Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan.

Authors:  Cherng-Lih Perng; Ming-Jr Jian; Chih-Kai Chang; Jung-Chung Lin; Kuo-Ming Yeh; Chien-Wen Chen; Sheng-Kang Chiu; Hsing-Yi Chung; Yi-Hui Wang; Shu-Jung Liao; Shih-Yi Li; Shan-Shan Hsieh; Shih-Hung Tsai; Feng-Yee Chang; Hung-Sheng Shang
Journal:  PeerJ       Date:  2020-06-17       Impact factor: 2.984

10.  Influx of multidrug-resistant organisms by country-to-country transfer of patients.

Authors:  Nico T Mutters; Frank Günther; Anja Sander; Alexander Mischnik; Uwe Frank
Journal:  BMC Infect Dis       Date:  2015-10-28       Impact factor: 3.090
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