Literature DB >> 27008873

Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.

Talita T Rocchetti1,2, Suzane Silbert3, Alicia Gostnell1, Carly Kubasek1, Raymond Widen1.   

Abstract

A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2016        PMID: 27008873      PMCID: PMC4879273          DOI: 10.1128/JCM.00241-16

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  27 in total

1.  Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii on the fully automated BD MAX platform.

Authors:  Alexander H Dalpke; Marjeta Hofko; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2013-05-15       Impact factor: 5.948

2.  Detection of Mycobacterium avium complex DNA directly in clinical respiratory specimens: opportunities for improved turn-around time and cost savings.

Authors:  Anh C Tran; Tanya A Halse; Vincent E Escuyer; Kimberlee A Musser
Journal:  Diagn Microbiol Infect Dis       Date:  2014-01-30       Impact factor: 2.803

3.  Evaluation of the MTBDRsl test for detection of second-line-drug resistance in Mycobacterium tuberculosis.

Authors:  Vo Sy Kiet; Nguyen Thi Ngoc Lan; Duong Duy An; Nguyen Huy Dung; Dai Viet Hoa; Nguyen van Vinh Chau; Nguyen Tran Chinh; Jeremy Farrar; Maxine Caws
Journal:  J Clin Microbiol       Date:  2010-06-23       Impact factor: 5.948

4.  Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR.

Authors:  Sung Jae Shin; Byung Soo Lee; Won-Jung Koh; Elizabeth J B Manning; Kelly Anklam; Srinand Sreevatsan; Randall S Lambrecht; Michael T Collins
Journal:  J Clin Microbiol       Date:  2010-09-01       Impact factor: 5.948

5.  Diagnosis of lymph node tuberculosis using the GeneXpert MTB/RIF in Tunisia.

Authors:  Asma Ghariani; Taha Jaouadi; Selma Smaoui; Emna Mehiri; Chama Marouane; Sana Kammoun; Leila Essalah; Maha Driss; Feriele Messadi; Leila Slim-Saidi
Journal:  Int J Mycobacteriol       Date:  2015-06-27

Review 6.  A systematic review of rapid diagnostic tests for the detection of tuberculosis infection.

Authors:  J Dinnes; J Deeks; H Kunst; A Gibson; E Cummins; N Waugh; F Drobniewski; A Lalvani
Journal:  Health Technol Assess       Date:  2007-01       Impact factor: 4.014

7.  Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children.

Authors:  E S Bruijnesteijn Van Coppenraet; J A Lindeboom; J M Prins; M F Peeters; E C J Claas; E J Kuijper
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

Review 8.  Pulmonary disease due to nontuberculous mycobacteria.

Authors:  Jeffrey Glassroth
Journal:  Chest       Date:  2008-01       Impact factor: 9.410

9.  In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.

Authors:  Laura L Flores; Madhukar Pai; John M Colford; Lee W Riley
Journal:  BMC Microbiol       Date:  2005-10-03       Impact factor: 3.605

10.  Retrospective study of the predictors of mortality and radiographic deterioration in 782 patients with nodular/bronchiectatic Mycobacterium avium complex lung disease.

Authors:  Mina Gochi; Noboru Takayanagi; Tetsu Kanauchi; Takashi Ishiguro; Tsutomu Yanagisawa; Yutaka Sugita
Journal:  BMJ Open       Date:  2015-08-05       Impact factor: 2.692

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  4 in total

1.  An alert of Mycobacterium tuberculosis infection of rhesus macaques in a wild zoo in China.

Authors:  Wenping Gong; Yourong Yang; Yi Luo; Ning Li; Xuejuan Bai; Yinping Liu; Junxian Zhang; Ming Chen; Chenglin Zhang; Xueqiong Wu
Journal:  Exp Anim       Date:  2017-06-29

2.  The Potential of Digital Polymerase Chain Reaction for Improving Diagnostic Yield of Nontuberculous Mycobacteria Pulmonary Disease.

Authors:  Yoichi Nishii; Kazuki Furuhashi; Saki Nakamura; Miho Nishio; Yuki Nakamura; Kengo Ushiro; Kentaro Ito; Tadashi Sakaguchi; Yuta Suzuki; Kentaro Fujiwara; Taro Yasuma; Tetsu Kobayashi; Corina D'Alessandro-Gabazza; Esteban C Gabazza; Osamu Taguchi; Osamu Hataji
Journal:  Infect Drug Resist       Date:  2021-12-01       Impact factor: 4.003

3.  Treatment Dilemmas in Disseminated Nontuberculous Mycobacterial Infections With Interferon-gamma Autoantibodies.

Authors:  Elizabeth M King; Victoria K Weaver; Mary H Kestler
Journal:  Open Forum Infect Dis       Date:  2021-06-23       Impact factor: 3.835

Review 4.  Advances in Molecular Diagnosis of Tuberculosis.

Authors:  Emily MacLean; Mikashmi Kohli; Stefan F Weber; Anita Suresh; Samuel G Schumacher; Claudia M Denkinger; Madhukar Pai
Journal:  J Clin Microbiol       Date:  2020-09-22       Impact factor: 5.948

  4 in total

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