| Literature DB >> 24510436 |
Brian J Beliveau1, Nicholas Apostolopoulos, Chao-ting Wu.
Abstract
Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation.Entities:
Keywords: FISH; Oligopaint; complex DNA library; oligonucleotide
Mesh:
Substances:
Year: 2014 PMID: 24510436 PMCID: PMC3928790 DOI: 10.1002/0471142727.mb1423s105
Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN: 1934-3647