Literature DB >> 24510436

Visualizing genomes with Oligopaint FISH probes.

Brian J Beliveau1, Nicholas Apostolopoulos, Chao-ting Wu.   

Abstract

Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation.
Copyright © 2014 John Wiley & Sons, Inc.

Entities:  

Keywords:  FISH; Oligopaint; complex DNA library; oligonucleotide

Mesh:

Substances:

Year:  2014        PMID: 24510436      PMCID: PMC3928790          DOI: 10.1002/0471142727.mb1423s105

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN: 1934-3647


  25 in total

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8.  Visualization of fine-scale genomic structure by oligonucleotide-based high-resolution FISH.

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7.  Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes.

Authors:  Brian J Beliveau; Alistair N Boettiger; Maier S Avendaño; Ralf Jungmann; Ruth B McCole; Eric F Joyce; Caroline Kim-Kiselak; Frédéric Bantignies; Chamith Y Fonseka; Jelena Erceg; Mohammed A Hannan; Hien G Hoang; David Colognori; Jeannie T Lee; William M Shih; Peng Yin; Xiaowei Zhuang; Chao-ting Wu
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9.  OligoMiner provides a rapid, flexible environment for the design of genome-scale oligonucleotide in situ hybridization probes.

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10.  In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT.

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