Literature DB >> 2450208

A fast and sensitive method for detecting specific viral RNA in mammalian cells.

U Paeratakul1, P R De Stasio, M W Taylor.   

Abstract

A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.

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Year:  1988        PMID： 2450208      PMCID： PMC253120     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  9 in total

1.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

Authors:  S Hayashi; I C Gillam; A D Delaney; G M Tener
Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

2.  In situ dot blots: quantitation of mRNA in intact cells.

Authors:  S M Yu; M A Gorovsky
Journal:  Nucleic Acids Res       Date:  1986-10-10       Impact factor: 16.971

3.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

4.  Mengovirus RNA synthesis in productive and restrictive cell lines.

Authors:  R Wall; M W Taylor
Journal:  Virology       Date:  1970-09       Impact factor: 3.616

5.  Induction of c-myc expression in human B lymphocytes by B-cell growth factor and anti-immunoglobulin.

Authors:  J Lacy; S N Sarkar; W C Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

6.  Accumulation of histone repeat transcripts in the sea urchin egg pronucleus.

Authors:  D L Venezky; L M Angerer; R C Angerer
Journal:  Cell       Date:  1981-05       Impact factor: 41.582

7.  Quick-blot: selective mRNA or DNA immobilization from whole cells.

Authors:  J Bresser; J Doering; D Gillespie
Journal:  DNA       Date:  1983

8.  Carcinogen-mediated induction of SV40 DNA synthesis in SV40 transformed Chinese hamster embryo cells.

Authors:  S Lavi; S Etkin
Journal:  Carcinogenesis       Date:  1981       Impact factor: 4.944

9.  The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region.

Authors:  A C Palmenberg; E M Kirby; M R Janda; N L Drake; G M Duke; K F Potratz; M S Collett
Journal:  Nucleic Acids Res       Date:  1984-03-26       Impact factor: 16.971
  9 in total
  19 in total

1.  trans rescue of a mutant poliovirus RNA polymerase function.

Authors:  W A Charini; C C Burns; E Ehrenfeld; B L Semler
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

2.  Analysis of interferon-gamma resistant mutants that are possibly defective in their signaling mechanism.

Authors:  G S Feng; W Dai; S L Gupta; G Werner-Felmayer; H Wachter; O Takikawa; M W Taylor
Journal:  Mol Gen Genet       Date:  1991-11

3.  Defective expression of adenovirus genes during early infection of undifferentiated OTF963 embryonal carcinoma cells.

Authors:  C C Nelson; A W Braithwaite; M Silvestro; A J Bellett
Journal:  J Virol       Date:  1990-09       Impact factor: 5.103

4.  Differentiation of respiratory syncytial virus subgroups with cDNA probes in a nucleic acid hybridization assay.

Authors:  W M Sullender; L J Anderson; K Anderson; G W Wertz
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

5.  Interferon gamma-resistant mutants are defective in the induction of indoleamine 2,3-dioxygenase.

Authors:  G S Feng; M W Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

6.  E1a-dependent expression of adenovirus genes in OTF963 embryonal carcinoma cells: role of E1a-induced differentiation.

Authors:  C C Nelson; A W Braithwaite; M Silvestro; A J Bellett
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

7.  Selected mutations of the duck hepatitis B virus P gene RNase H domain affect both RNA packaging and priming of minus-strand DNA synthesis.

Authors:  Y Chen; W S Robinson; P L Marion
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103

8.  Protective effect of interferon beta on human T cell leukaemia virus type I infection of CD4+ T cells isolated from human cord blood.

Authors:  B Macchi; I Faraoni; A Mastino; C D'Onofrio; G Romeo; E Bonmassar
Journal:  Cancer Immunol Immunother       Date:  1993-07       Impact factor: 6.968

9.  Liposome-mediated transfection of intact viral particles reveals that plasma membrane penetration determines permissivity of tissue culture cells to rotavirus.

Authors:  D M Bass; M R Baylor; C Chen; E M Mackow; M Bremont; H B Greenberg
Journal:  J Clin Invest       Date:  1992-12       Impact factor: 14.808

10.  Expression of nonstructural protein NS3 of African horsesickness virus (AHSV): evidence for a cytotoxic effect of NS3 in insect cells, and characterization of the gene products in AHSV infected Vero cells.

Authors:  V van Staden; M A Stoltz; H Huismans
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574
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