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Literature DB >> 2449452

Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand.

S Kasempimolporn¹, S Louisirirotchanakul, P Sinarachatanant, C Wasi.

Abstract

Detection of rotavirus by polyacrylamide gel electrophoresis in combination with silver staining and by enzyme-linked immunosorbent assay showed 96.7% identical results in tests with 1,304 stool specimens from diarrheic patients. The polyacrylamide gel electrophoresis method can be modified to reduce cost and working time. Phenol extraction of stools, however, is essential in maintaining the sensitivity of the method.

Entities: Chemical Species

Mesh：

Substances：
RNA, Viral
Silver

Year: 1988 PMID： 2449452 PMCID： PMC266235 DOI： 10.1128/jcm.26.1.158-160.1988

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. Human rotavirus and its antibody: their coexistence in feces of infants.

Authors: H Watanabe; I D Gust; I H Holmes
Journal: J Clin Microbiol Date: 1978-05 Impact factor: 5.948

2. Solid-phase radioimmunoassay for the detection of rotavirus.

Authors: P J Middleton; M D Holdaway; M Petric; M T Szymanski; J S Tam
Journal: Infect Immun Date: 1977-05 Impact factor: 3.441

3. Epidemiology of rotavirus electropherotypes determined by a simplified diagnostic technique with RNA analysis.

Authors: K T Dolan; E M Twist; P Horton-Slight; C Forrer; L M Bell; S A Plotkin; H F Clark
Journal: J Clin Microbiol Date: 1985-05 Impact factor: 5.948

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors: U E Loening
Journal: Biochem J Date: 1967-01 Impact factor: 3.857

6. The epidemiological study on viral diarrhoea in Thailand.

Authors: C Wasi; S Louisirirotchanakul; K Thakerngpol; S Satrasook; M Surakhala; W Varavithya; P Thongcharoen
Journal: J Med Assoc Thai Date: 1984-07

7. Attachment of SA-11 rotavirus to erythrocyte receptors.

Authors: J W Bastardo; I H Holmes
Journal: Infect Immun Date: 1980-09 Impact factor: 3.441

8. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

Authors: A J Herring; N F Inglis; C K Ojeh; D R Snodgrass; J D Menzies
Journal: J Clin Microbiol Date: 1982-09 Impact factor: 5.948

Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand.

1. Human rotavirus and its antibody: their coexistence in feces of infants.

2. Solid-phase radioimmunoassay for the detection of rotavirus.

3. Epidemiology of rotavirus electropherotypes determined by a simplified diagnostic technique with RNA analysis.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

6. The epidemiological study on viral diarrhoea in Thailand.

7. Attachment of SA-11 rotavirus to erythrocyte receptors.

8. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

9. Etiologic studies of the 1983 and 1984 outbreaks of epidemic diarrhea in Guangxi.

10. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.

1. Rapid screening test for the diagnosis of rotavirus infection.

2. Molecular epidemiology of rotaviruses associated with pediatric diarrhea in Bangkok, Thailand.

3. Occurrence of nonspecific reactions among stool specimens tested by the Abbott TestPack rotavirus enzyme immunoassay.

4. Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype.

5. Diagnosis of rotavirus infection.