Literature DB >> 6182158

Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

A J Herring, N F Inglis, C K Ojeh, D R Snodgrass, J D Menzies.   

Abstract

A rapid simple technique for the diagnosis of rotavirus has been developed based on the sensitive detection of rotavirus double-stranded RNA genome segments separated in polyacrylamide gels. The method utilizes a recently described ultrasensitive silver stain for polypeptides, which can also detect subnanogram amounts of nucleic acid. The sensitivity of the technique is comparable with that of electron microscopy or enzyme-linked immunosorbent assay.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6182158      PMCID： PMC272392          DOI： 10.1128/jcm.16.3.473-477.1982

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-09       Impact factor: 5.948

2.  Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

3.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

4.  Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.

Authors:  C R Merril; D Goldman; S A Sedman; M H Ebert
Journal:  Science       Date:  1981-03-27       Impact factor: 47.728

5.  Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2.

Authors:  A R Kalica; H B Greenberg; R T Espejo; J Flores; R G Wyatt; A Z Kapikian; R M Chanock
Journal:  Infect Immun       Date:  1981-09       Impact factor: 3.441

6.  A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus.

Authors:  I N Clarke; M A McCrae
Journal:  J Virol Methods       Date:  1981-03       Impact factor: 2.014

7.  Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences.

Authors:  R T Espejo; O Muñóz; F Serafin; P Romero
Journal:  Infect Immun       Date:  1980-02       Impact factor: 3.441

8.  The classification and nomenclature of viruses. Summary of results of meetings of the International Committee on Taxonomy of Viruses in The Hague, September 1978.

Authors:  R E Matthews
Journal:  Intervirology       Date:  1979       Impact factor: 1.763

9.  Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs.

Authors:  L J Saif; E H Bohl; K W Theil; R F Cross; J A House
Journal:  J Clin Microbiol       Date:  1980-07       Impact factor: 5.948

10.  Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid.

Authors:  S M Rodger; R F Bishop; C Birch; B McLean; I H Holmes
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948
View more
  230 in total

1.  Rotavirus G and P genotypes in rural Ghana.

Authors:  R H Asmah; J Green; G E Armah; C I Gallimore; J J Gray; M Iturriza-Gómara; F Anto; A Oduro; F N Binka; D W Brown; F Cutts
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

2.  Molecular characterization of rotavirus in Ireland: detection of novel strains circulating in the population.

Authors:  F O'Halloran; M Lynch; B Cryan; H O'Shea; S Fanning
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

3.  Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: emergence of type G9 in 1995.

Authors:  L E Unicomb; G Podder; J R Gentsch; P A Woods; K Z Hasan; A S Faruque; M J Albert; R I Glass
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

4.  Antigenic and genetic characterization of serotype G2 human rotavirus strains from the African continent.

Authors:  N A Page; A D Steele
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

5.  Evidence for two serotype G3 subtypes among equine rotaviruses.

Authors:  G F Browning; R M Chalmers; T A Fitzgerald; D R Snodgrass
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948

6.  Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe.

Authors:  J Fernández; A Sandino; A Yudelevich; L F Avendaño; A Venegas; V Hinrichsen; E Spencer
Journal:  Epidemiol Infect       Date:  1992-02       Impact factor: 2.451

7.  Genotyping of enteric adenoviruses by using single-stranded conformation polymorphism analysis and heteroduplex mobility assay.

Authors:  Caroline C Soares; Eduardo M Volotão; Maria Carolina M Albuquerque; Carlos M Nozawa; Rosa Elisa C Linhares; Dmitriy Volokhov; Vladimir Chizhikov; Xiaoyan Lu; Dean Erdman; Norma Santos
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

8.  Growth and characterisation of human faecal astrovirus in a continuous cell line.

Authors:  M M Willcocks; M J Carter; F R Laidler; C R Madeley
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

9.  Prospective study of community-acquired rotavirus infection.

Authors:  B J Zheng; S K Lo; J S Tam; M Lo; C Y Yeung; M H Ng
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

10.  Temporal and geographical distributions of human rotavirus serotypes, 1983 to 1988.

Authors:  G M Beards; U Desselberger; T H Flewett
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.