Literature DB >> 863510

Solid-phase radioimmunoassay for the detection of rotavirus.

P J Middleton, M D Holdaway, M Petric, M T Szymanski, J S Tam.   

Abstract

A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. In a field study on stool specimens it was at least as efficient as electron microscopy.

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Year:  1977        PMID： 863510      PMCID： PMC420969          DOI： 10.1128/iai.16.2.439-444.1977

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  11 in total

1.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors:  F C GREENWOOD; W M HUNTER; J S GLOVER
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

2.  Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody.

Authors:  P J Middleton; M Petric; C M Hewitt; M T Szymanski; J S Tam
Journal:  J Clin Pathol       Date:  1976-03       Impact factor: 3.411

3.  Radioimmunoassay for herpes simplex virus.

Authors:  L V dos Remedios; P M Weber; L Drew
Journal:  J Immunol Methods       Date:  1976       Impact factor: 2.303

4.  Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.

Authors:  K O Kalimo; O H Meurman; P E Halonen; B R Ziola; M K Viljanen; K Granfors; P Toivanen
Journal:  J Clin Microbiol       Date:  1976-08       Impact factor: 5.948

5.  Antibody-coated tube method for radioimmunoassay of human growth hormone.

Authors:  K J Catt; G W Tregear; H G Burger; C Skermer
Journal:  Clin Chim Acta       Date:  1970-02       Impact factor: 3.786

6.  Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with the passive hemagglutination methods.

Authors:  F B Hollinger; V Vorndam; G R Dreesman
Journal:  J Immunol       Date:  1971-10       Impact factor: 5.422

7.  Solid-phase radioimmunoassay of total and influenza-specific immunoglobulin G.

Authors:  H Daugharty; D T Warfield; M L Davis
Journal:  Appl Microbiol       Date:  1972-02

8.  Purification and preliminary characterization of infantile gastroenteritis virus (orbivirus group).

Authors:  M Petric; M T Szymanski; P J Middleton
Journal:  Intervirology       Date:  1975       Impact factor: 1.763

9.  A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.

Authors:  R H Purcell; D C Wong; Y Moritsugu; J L Dienstag; J A Routenberg; J D Boggs
Journal:  J Immunol       Date:  1976-02       Impact factor: 5.422

10.  An enzymic method for the trace iodination of immunoglobulins and other proteins.

Authors:  J J Marchalonis
Journal:  Biochem J       Date:  1969-06       Impact factor: 3.857
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  27 in total

1.  Detection of rotavirus from faeces by reversed passive haemagglutination method.

Authors:  T Sanekata; Y Yoshida; K Oda
Journal:  J Clin Pathol       Date:  1979-09       Impact factor: 3.411

2.  Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.

Authors:  C J Birch; N I Lehmann; A J Hawker; J A Marshall; I D Gust
Journal:  J Clin Pathol       Date:  1979-07       Impact factor: 3.411

3.  Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

4.  RNA polymerase associated with human rotaviruses in diarrhea stools.

Authors:  J F Hruska; M F Notter; M A Menegus; M C Steinhoff
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

5.  Human rotavirus and its antibody: their coexistence in feces of infants.

Authors:  H Watanabe; I D Gust; I H Holmes
Journal:  J Clin Microbiol       Date:  1978-05       Impact factor: 5.948

6.  Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens.

Authors:  P H Dennehy; D R Gauntlett; W E Tente
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

7.  Examination of the Rotazyme II enzyme immunoassay for the diagnosis of rotavirus gastroenteritis.

Authors:  M Chernesky; S Castriciano; J Mahony; D DeLong
Journal:  J Clin Microbiol       Date:  1985-09       Impact factor: 5.948

8.  Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.

Authors:  D J Ellens; P W de Leeuw; P J Straver; J A van Balken
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402

9.  Comparison of results using electron microscope, immunodiffusion and fluorescent antibody analyses to detect rotavirus in diarrheic fecal samples of calves.

Authors:  M B Rhodes; E L Stair; R A McCullough; L D McGill; C A Mebus
Journal:  Can J Comp Med       Date:  1979-01

10.  Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.

Authors:  H K Sarkkinen; P E Halonen; P P Arstila; A A Salmi
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948
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