| Literature DB >> 24486374 |
Krista A Shisler1, Joan B Broderick2.
Abstract
The glycyl radical enzyme activating enzymes (GRE-AEs) are a group of enzymes that belong to the radical S-adenosylmethionine (SAM) superfamily and utilize a [4Fe-4S] cluster and SAM to catalyze H-atom abstraction from their substrate proteins. GRE-AEs activate homodimeric proteins known as glycyl radical enzymes (GREs) through the production of a glycyl radical. After activation, these GREs catalyze diverse reactions through the production of their own substrate radicals. The GRE-AE pyruvate formate lyase activating enzyme (PFL-AE) is extensively characterized and has provided insights into the active site structure of radical SAM enzymes including GRE-AEs, illustrating the nature of the interactions with their corresponding substrate GREs and external electron donors. This review will highlight research on PFL-AE and will also discuss a few GREs and their respective activating enzymes.Entities:
Keywords: Glycyl radical enzyme (GRE); Glycyl radical enzyme activating enzyme (GRE–AE); Pyruvate formate lyase activating enzyme (PFL-AE); Radical S-adenosylmethionine (SAM) enzyme
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Year: 2014 PMID： 24486374 PMCID： PMC4083501 DOI： 10.1016/j.abb.2014.01.020
Source DB: PubMed Journal: Arch Biochem Biophys ISSN： 0003-9861 Impact factor: 4.013