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Literature DB >> 2447957

Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants.

Abstract

Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of E. coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.

Entities: Chemical Gene Species

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Year: 1987 PMID： 2447957 DOI： 10.1016/0300-9084(87)90216-1

Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079

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Cited

35 in total

1. In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications.

Authors: H Moine; C L Squires; B Ehresmann; C Ehresmann
Journal: Proc Natl Acad Sci U S A Date: 2000-01-18 Impact factor: 11.205

2. Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.

Authors: C D Prescott; H C Kornau
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

3. Antibiotic resistance markers for genetic manipulations of Leptospira spp.

Authors: Dante Poggi; Priscila Oliveira de Giuseppe; Mathieu Picardeau
Journal: Appl Environ Microbiol Date: 2010-05-28 Impact factor: 4.792

4. The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits.

Authors: J Mattias Lövgren; Göran O Bylund; Manoj K Srivastava; L A Carina Lundberg; Olof P Persson; Gunnar Wingsle; P Mikael Wikström
Journal: RNA Date: 2004-11 Impact factor: 4.942

5. A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity.

Authors: Narayanaswamy Kirthi; Biswajoy Roy-Chaudhuri; Teresa Kelley; Gloria M Culver
Journal: RNA Date: 2006-10-19 Impact factor: 4.942

6. Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida.

Authors: Corinna Kehrenberg; Stefan Schwarz
Journal: Antimicrob Agents Chemother Date: 2007-03-19 Impact factor: 5.191

Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants.

1. In vivo selection of functional ribosomes with variations in the rRNA-binding site of Escherichia coli ribosomal protein S8: evolutionary implications.

2. Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.

3. Antibiotic resistance markers for genetic manipulations of Leptospira spp.

4. The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits.

5. A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity.

6. Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida.

7. Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi.

8. Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.

9. Pseudoknot in the central domain of small subunit ribosomal RNA is essential for translation.

10. Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.