Literature DB >> 1374555

Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.

C D Prescott1, H C Kornau.   

Abstract

The in vivo expression of mutations constructed within helix 34 of 16S rRNA has been examined together with a nonsense tRNA suppressor for their action at stop codons. The data revealed two novel results: in contrast to previous findings, some of the rRNA mutations affected suppression at UAA and UAG nonsense codons. Secondly, both an increase and a decrease in the efficiency of the suppressor tRNA were induced by the mutations. This is the first report that rRNA mutations decreased the efficiency of a suppressor tRNA. The data are interpreted as there being competition between the two release factors (RF-1 and RF-2) for an overlapping domain and that helix 34 influences this interaction.

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Year:  1992        PMID： 1374555      PMCID： PMC312239          DOI： 10.1093/nar/20.7.1567

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  A single mutation in 16S rRNA that affects mRNA binding and translation-termination.

Authors:  C D Prescott; H U Göringer
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

2.  The involvement of base 1054 in 16S rRNA for UGA stop codon dependent translational termination.

Authors:  A Hänfler; B Kleuvers; H U Göringer
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

3.  A rRNA-mRNA base pairing model for UGA-dependent termination.

Authors:  C D Prescott; B Kleuvers; H U Göringer
Journal:  Biochimie       Date:  1991 Jul-Aug       Impact factor: 4.079

4.  Ribosomes containing the C1054-deletion mutation in E. coli 16S rRNA act as suppressors at all three nonsense codons.

Authors:  C Prescott; L Krabben; K Nierhaus
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

5.  Beta-lactamase assays.

Authors:  G W Ross; C H O'Callaghan
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

6.  Interaction of the release factor with the Escherichia coli ribosome: inhibition of the 30S subunit domain by specific antibodies.

Authors:  W P Tate; K K McCaughan; B Kastner; C N Trotman; M Stoffler-Meilicke; G Stoffler
Journal:  Biochem Int       Date:  1988-07

7.  Rapid chemical probing of conformation in 16 S ribosomal RNA and 30 S ribosomal subunits using primer extension.

Authors:  D Moazed; S Stern; H F Noller
Journal:  J Mol Biol       Date:  1986-02-05       Impact factor: 5.469

8.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.

Authors:  J Brosius; T J Dull; D D Sleeter; H F Noller
Journal:  J Mol Biol       Date:  1981-05-15       Impact factor: 5.469

9.  Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs.

Authors:  L A Raftery; J B Egan; S W Cline; M Yarus
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  Mutant 16S ribosomal RNA: a codon-specific translational suppressor.

Authors:  E J Murgola; K A Hijazi; H U Göringer; A E Dahlberg
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205
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  6 in total

Review 1.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 2.  Throwing a spanner in the works: antibiotics and the translation apparatus.

Authors:  C M Spahn; C D Prescott
Journal:  J Mol Med (Berl)       Date:  1996-08       Impact factor: 4.599

3.  Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome.

Authors:  A B Matassova; M V Rodnina; W Wintermeyer
Journal:  RNA       Date:  2001-12       Impact factor: 4.942

4.  The translational function of nucleotide C1054 in the small subunit rRNA is conserved throughout evolution: genetic evidence in yeast.

Authors:  Y O Chernoff; G P Newnam; S W Liebman
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-19       Impact factor: 11.205

5.  Two regions of the Escherichia coli 16S ribosomal RNA are important for decoding stop signals in polypeptide chain termination.

Authors:  C M Brown; K K McCaughan; W P Tate
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

6.  Distinct functional classes of ram mutations in 16S rRNA.

Authors:  Sean P McClory; Aishwarya Devaraj; Kurt Fredrick
Journal:  RNA       Date:  2014-02-26       Impact factor: 4.942
  6 in total

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