Literature DB >> 24478508

A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus.

Janessa Pickering1, Michael J Binks, Jemima Beissbarth, Kim M Hare, Lea-Ann S Kirkham, Heidi Smith-Vaughan.   

Abstract

We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.

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Year:  2013        PMID： 24478508      PMCID： PMC3911354          DOI： 10.1128/JCM.02191-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Detection of bacterial pathogens in Mongolia meningitis surveillance with a new real-time PCR assay to detect Haemophilus influenzae.

Authors:  Xin Wang; Raydel Mair; Cynthia Hatcher; M Jordan Theodore; Karen Edmond; Henry M Wu; Brian H Harcourt; Maria da Gloria S Carvalho; Fabiana Pimenta; Pagbajab Nymadawa; Dorjpurev Altantsetseg; Mariah Kirsch; Sarah W Satola; Amanda Cohn; Nancy E Messonnier; Leonard W Mayer
Journal:  Int J Med Microbiol       Date:  2011-01-26       Impact factor: 3.473

2.  Impact of the 10-valent pneumococcal non-typeable Haemophilus influenzae Protein D conjugate vaccine (PHiD-CV) on bacterial nasopharyngeal carriage.

Authors:  Roman Prymula; Irena Hanovcova; Miroslav Splino; Pavla Kriz; Jitka Motlova; Vera Lebedova; Patricia Lommel; Eva Kaliskova; Thierry Pascal; Dorota Borys; Lode Schuerman
Journal:  Vaccine       Date:  2011-01-06       Impact factor: 3.641

3.  Effect of vaccination with pneumococcal capsular polysaccharides conjugated to Haemophilus influenzae-derived protein D on nasopharyngeal carriage of Streptococcus pneumoniae and H. influenzae in children under 2 years of age.

Authors:  Roman Prymula; Pavla Kriz; Eva Kaliskova; Thierry Pascal; Jan Poolman; Lode Schuerman
Journal:  Vaccine       Date:  2009-10-08       Impact factor: 3.641

Review 4.  Role of nontypeable Haemophilus influenzae in otitis media and chronic obstructive pulmonary disease.

Authors:  Yasmin Thanavala; Amit A Lugade
Journal:  Adv Otorhinolaryngol       Date:  2011-08-18

5.  Nontypeable Haemophilus influenzae invasive disease in The Netherlands: a retrospective surveillance study 2001-2008.

Authors:  Karen van Wessel; Gerwin D Rodenburg; Reinier H Veenhoven; Lodewijk Spanjaard; Arie van der Ende; Elisabeth A M Sanders
Journal:  Clin Infect Dis       Date:  2011-07-01       Impact factor: 9.079

6.  Current epidemiology and trends in invasive Haemophilus influenzae disease--United States, 1989-2008.

Authors:  Jessica R MacNeil; Amanda C Cohn; Monica Farley; Raydel Mair; Joan Baumbach; Nancy Bennett; Ken Gershman; Lee H Harrison; Ruth Lynfield; Susan Petit; Arthur Reingold; William Schaffner; Ann Thomas; Fatima Coronado; Elizabeth R Zell; Leonard W Mayer; Thomas A Clark; Nancy E Messonnier
Journal:  Clin Infect Dis       Date:  2011-12       Impact factor: 9.079

7.  Nasopharyngeal carriage of Haemophilus haemolyticus in otitis-prone and healthy children.

Authors:  Lea-Ann S Kirkham; Selma P Wiertsema; Eva N Mowe; Jacinta M Bowman; Thomas V Riley; Peter C Richmond
Journal:  J Clin Microbiol       Date:  2010-05-12       Impact factor: 5.948

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Comparison of laboratory-based and phylogenetic methods to distinguish between Haemophilus influenzae and H. haemolyticus.

Authors:  Sara A Sandstedt; Lixin Zhang; Mayurika Patel; Kirk W McCrea; Zhaohui Qin; Carl F Marrs; Janet R Gilsdorf
Journal:  J Microbiol Methods       Date:  2008-07-03       Impact factor: 2.363

10.  Effects of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D-conjugate vaccine on nasopharyngeal bacterial colonization in young children: a randomized controlled trial.

Authors:  Menno R van den Bergh; Judith Spijkerman; Kristien M Swinnen; Nancy A François; Thierry G Pascal; Dorota Borys; Lode Schuerman; Ed P F Ijzerman; Jacob P Bruin; Arie van der Ende; Reinier H Veenhoven; Elisabeth A M Sanders
Journal:  Clin Infect Dis       Date:  2012-11-01       Impact factor: 9.079
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  11 in total

1.  Rapid Differentiation of Haemophilus influenzae and Haemophilus haemolyticus by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry with ClinProTools Mass Spectrum Analysis.

Authors:  Jonathan H K Chen; Vincent C C Cheng; Chun-Pong Wong; Sally C Y Wong; Wing-Cheong Yam; Kwok-Yung Yuen
Journal:  J Clin Microbiol       Date:  2017-06-21       Impact factor: 5.948

2.  Comparative Genomic Analysis of Haemophilus haemolyticus and Nontypeable Haemophilus influenzae and a New Testing Scheme for Their Discrimination.

Authors:  Fang Hu; Lavanya Rishishwar; Ambily Sivadas; Gabriel J Mitchell; I King Jordan; Timothy F Murphy; Janet R Gilsdorf; Leonard W Mayer; Xin Wang
Journal:  J Clin Microbiol       Date:  2016-10-05       Impact factor: 5.948

3.  Complete Deletion of the Fucose Operon in Haemophilus influenzae Is Associated with a Cluster in Multilocus Sequence Analysis-Based Phylogenetic Group II Related to Haemophilus haemolyticus: Implications for Identification and Typing.

Authors:  Camilla de Gier; Lea-Ann S Kirkham; Niels Nørskov-Lauritsen
Journal:  J Clin Microbiol       Date:  2015-09-16       Impact factor: 5.948

4.  Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains.

Authors:  Camilla de Gier; Janessa L Pickering; Peter C Richmond; Ruth B Thornton; Lea-Ann S Kirkham
Journal:  J Clin Microbiol       Date:  2016-06-22       Impact factor: 5.948

5.  Haemophilus influenzae: using comparative genomics to accurately identify a highly recombinogenic human pathogen.

Authors:  Erin P Price; Derek S Sarovich; Elizabeth Nosworthy; Jemima Beissbarth; Robyn L Marsh; Janessa Pickering; Lea-Ann S Kirkham; Anthony D Keil; Anne B Chang; Heidi C Smith-Vaughan
Journal:  BMC Genomics       Date:  2015-08-27       Impact factor: 3.969

Review 6.  Molecular tools for differentiation of non-typeable Haemophilus influenzae from Haemophilus haemolyticus.

Authors:  Janessa Pickering; Peter C Richmond; Lea-Ann S Kirkham
Journal:  Front Microbiol       Date:  2014-12-02       Impact factor: 5.640

7.  Children with chronic suppurative lung disease have a reduced capacity to synthesize interferon-gamma in vitro in response to non-typeable Haemophilus influenzae.

Authors:  Susan J Pizzutto; Stephanie T Yerkovich; John W Upham; Belinda J Hales; Wayne R Thomas; Anne B Chang
Journal:  PLoS One       Date:  2014-08-11       Impact factor: 3.240

8.  Haemophilus haemolyticus Interaction with Host Cells Is Different to Nontypeable Haemophilus influenzae and Prevents NTHi Association with Epithelial Cells.

Authors:  Janessa L Pickering; Amy Prosser; Karli J Corscadden; Camilla de Gier; Peter C Richmond; Guicheng Zhang; Ruth B Thornton; Lea-Ann S Kirkham
Journal:  Front Cell Infect Microbiol       Date:  2016-05-09       Impact factor: 5.293

9.  Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis.

Authors:  Jemima Beissbarth; Michael J Binks; Robyn L Marsh; Anne B Chang; Amanda J Leach; Heidi C Smith-Vaughan
Journal:  BMC Res Notes       Date:  2018-05-21

10.  Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR).

Authors:  Andrew Turner; Jurgen Sasse; Aniko Varadi
Journal:  BMC Med Genet       Date:  2016-10-19       Impact factor: 2.103
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