Literature DB >> 27335148

Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains.

Camilla de Gier1, Janessa L Pickering2, Peter C Richmond3, Ruth B Thornton2, Lea-Ann S Kirkham4.   

Abstract

We have developed a specific Haemophilus influenzae quantitative PCR (qPCR) that also identifies fucose-negative and protein D-negative strains. Analysis of 100 H. influenzae isolates, 28 Haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (P < 0.001).
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2016        PMID： 27335148      PMCID： PMC5005495          DOI： 10.1128/JCM.00982-16

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Detection of bacterial pathogens in Mongolia meningitis surveillance with a new real-time PCR assay to detect Haemophilus influenzae.

Authors:  Xin Wang; Raydel Mair; Cynthia Hatcher; M Jordan Theodore; Karen Edmond; Henry M Wu; Brian H Harcourt; Maria da Gloria S Carvalho; Fabiana Pimenta; Pagbajab Nymadawa; Dorjpurev Altantsetseg; Mariah Kirsch; Sarah W Satola; Amanda Cohn; Nancy E Messonnier; Leonard W Mayer
Journal:  Int J Med Microbiol       Date:  2011-01-26       Impact factor: 3.473

2.  Nonencapsulated or nontypeable Haemophilus influenzae are more likely than their encapsulated or serotypeable counterparts to have mutations in their fucose operon.

Authors:  Michelle L Shuel; Kathleen E Karlowsky; Dennis K S Law; Raymond S W Tsang
Journal:  Can J Microbiol       Date:  2011-11-22       Impact factor: 2.419

Review 3.  Infection in the pathogenesis and course of chronic obstructive pulmonary disease.

Authors:  Sanjay Sethi; Timothy F Murphy
Journal:  N Engl J Med       Date:  2008-11-27       Impact factor: 91.245

4.  Haemophilus influenzae outer membrane protein P6 molecular characterization may not differentiate all strains of H. Influenzae from H. haemolyticus.

Authors:  Arthur Chang; Diana G Adlowitz; Edna Yellamatty; Michael Pichichero
Journal:  J Clin Microbiol       Date:  2010-08-04       Impact factor: 5.948

5.  Frequency of recovery of pathogens causing acute maxillary sinusitis in adults before and after introduction of vaccination of children with the 7-valent pneumococcal vaccine.

Authors:  Itzhak Brook; Perry A Foote; Jeffrey N Hausfeld
Journal:  J Med Microbiol       Date:  2006-07       Impact factor: 2.472

6.  Detection of cryptic genospecies misidentified as Haemophilus influenzae in routine clinical samples by assessment of marker genes fucK, hap, and sodC.

Authors:  Niels Nørskov-Lauritsen
Journal:  J Clin Microbiol       Date:  2009-06-17       Impact factor: 5.948

Review 7.  Non-typeable Haemophilus influenzae, an under-recognised pathogen.

Authors:  Johan Van Eldere; Mary P E Slack; Shamez Ladhani; Allan W Cripps
Journal:  Lancet Infect Dis       Date:  2014-07-07       Impact factor: 25.071

8.  Delineation of the species Haemophilus influenzae by phenotype, multilocus sequence phylogeny, and detection of marker genes.

Authors:  Niels Nørskov-Lauritsen; Merete D Overballe; Mogens Kilian
Journal:  J Bacteriol       Date:  2008-12-05       Impact factor: 3.490

9.  Molecular surveillance of true nontypeable Haemophilus influenzae: an evaluation of PCR screening assays.

Authors:  Michael J Binks; Beth Temple; Lea-Ann Kirkham; Selma P Wiertsema; Eileen M Dunne; Peter C Richmond; Robyn L Marsh; Amanda J Leach; Heidi C Smith-Vaughan
Journal:  PLoS One       Date:  2012-03-28       Impact factor: 3.240

10.  Haemophilus influenzae: using comparative genomics to accurately identify a highly recombinogenic human pathogen.

Authors:  Erin P Price; Derek S Sarovich; Elizabeth Nosworthy; Jemima Beissbarth; Robyn L Marsh; Janessa Pickering; Lea-Ann S Kirkham; Anthony D Keil; Anne B Chang; Heidi C Smith-Vaughan
Journal:  BMC Genomics       Date:  2015-08-27       Impact factor: 3.969
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  7 in total

1.  Differences in Pneumococcal and Haemophilus influenzae Natural Antibody Development in Papua New Guinean Children in the First Year of Life.

Authors:  Kelly M Martinovich; Tasmina Rahman; Camilla de Gier; Elke J Seppanen; Tilda Orami; Caitlyn M Granland; Jacinta Francis; Mition Yoannes; Karli J Corscadden; Rebecca Ford; Peter Jacoby; Anita H J van den Biggelaar; Lauren O Bakaletz; Allan W Cripps; Deborah Lehmann; Peter C Richmond; William S Pomat; Lea-Ann S Kirkham; Ruth B Thornton
Journal:  Front Immunol       Date:  2021-08-10       Impact factor: 7.561

2.  Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.

Authors:  Daya Marasini; Melissa J Whaley; Laurel T Jenkins; Fang Hu; Wenxin Jiang; Nadav Topaz; Alex Chen; Susanna Schmink; Jennifer Dolan Thomas; Brian H Harcourt; Henju Marjuki; Xin Wang
Journal:  J Clin Microbiol       Date:  2022-03-21       Impact factor: 11.677

3.  Haemophilus influenzae persists in biofilm communities in a smoke-exposed ferret model of COPD.

Authors:  Benjamin C Hunt; Denise Stanford; Xin Xu; Jindong Li; Amit Gaggar; Steven M Rowe; S Vamsee Raju; W Edward Swords
Journal:  ERJ Open Res       Date:  2020-08-11

4.  Australian Aboriginal Children with Otitis Media Have Reduced Antibody Titers to Specific Nontypeable Haemophilus influenzae Vaccine Antigens.

Authors:  Ruth B Thornton; Lea-Ann S Kirkham; Karli J Corscadden; Selma P Wiertsema; Angela Fuery; B Jan Jones; Harvey L Coates; Shyan Vijayasekaran; Guicheng Zhang; Anthony Keil; Peter C Richmond
Journal:  Clin Vaccine Immunol       Date:  2017-04-05

5.  Sleep Disordered Breathing and Recurrent Tonsillitis Are Associated With Polymicrobial Bacterial Biofilm Infections Suggesting a Role for Anti-Biofilm Therapies.

Authors:  Tulia Mateus; Elke J Seppanen; Camilla de Gier; Sharon Clark; Harvey Coates; Shyan Vijayasekaran; Karen Prosser; Selma P Wiertsema; Angela Fuery; Lea-Ann S Kirkham; Peter C Richmond; Ruth B Thornton
Journal:  Front Cell Infect Microbiol       Date:  2022-02-28       Impact factor: 5.293

6.  Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species.

Authors:  Karen L Osman; Johanna M C Jefferies; Christopher H Woelk; Nathalie Devos; Thierry G Pascal; Marie-Cécile Mortier; Jeanne-Marie Devaster; Tom M A Wilkinson; David W Cleary; Stuart C Clarke
Journal:  Sci Rep       Date:  2018-10-03       Impact factor: 4.379

7.  PCV7- and PCV10-Vaccinated Otitis-Prone Children in New Zealand Have Similar Pneumococcal and Haemophilus influenzae Densities in Their Nasopharynx and Middle Ear.

Authors:  Camilla de Gier; Caitlyn M Granland; Janessa L Pickering; Tony Walls; Mejbah Bhuiyan; Nikki Mills; Peter C Richmond; Emma J Best; Ruth B Thornton; Lea-Ann S Kirkham
Journal:  Vaccines (Basel)       Date:  2019-01-31
  7 in total

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