Literature DB >> 24426171

Development of Loop-Mediated Isothermal Amplification (LAMP) for Universal Detection of Enteroviruses.

Hua-Bing Zhao1, Guang-Ya Yin1, Guo-Ping Zhao1, Ai-Hua Huang1, Jun-Hong Wang1, Shuang-Feng Yang1, Hong-Sheng Gao1, Wei-Jun Kang2.   

Abstract

Enteroviruses are found in most environments and cause several diseases in humans. Loop-mediated isothermal amplification (LAMP) was adapted and evaluated for the rapid detection of enteroviruses. Based on the highly conserved 5' untranslated region (5'-UTR) of the human enteroviruses (HEVs), particularly human enterovirus A (HEV-A) and HEV-B, a set of universal primers was designed. The LAMP amplification was carried out under isothermal conditions at 61 °C, depending on the template concentration results were obtained within 45-90 min. The detection limits were found to be 10(1) copies of cloned enterovirus 71 fragments, more sensitive than conventional PCR. Nine water samples collected from drinking water sources during three seasons and 19 stool specimens collected from HFMD patients were analyzed. By using the LAMP assay, the majority of samples was tested positive, 9/9 (100 %) and 18/19 (94.7 %), respectively. LAMP is a practical method for the rapid detection of enteroviruses in environmental and clinical samples.

Entities:  

Keywords:  Detection; Drinking water source; Enterovirus; HFMD; LAMP

Year:  2013        PMID： 24426171      PMCID： PMC3889841          DOI： 10.1007/s12088-013-0399-7

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  19 in total

1.  Loop-mediated isothermal amplification of DNA.

Authors:  T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  Molecular classification of coxsackie A viruses on the basis of the 5'-UTR: structural and evolutionary aspects.

Authors:  Nikolaos Siafakas; Panayotis Markoulatos; Glyn Stanway
Journal:  J Mol Evol       Date:  2002-12       Impact factor: 2.395

3.  Enterovirus surveillance--United States, 1970-2005.

Authors:  Nino Khetsuriani; Ashley Lamonte-Fowlkes; Steven Oberst; Mark A Pallansch
Journal:  MMWR Surveill Summ       Date:  2006-09-15

4.  Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Authors:  Xiang Wang; Jun-ping Zhu; Qian Zhang; Zi-gang Xu; Fang Zhang; Zhi-hui Zhao; Wen-zhi Zheng; Li-shu Zheng
Journal:  J Virol Methods       Date:  2011-12-03       Impact factor: 2.014

Review 5.  Health risks of enteric viral infections in children.

Authors:  Nena Nwachuku; Charles P Gerba
Journal:  Rev Environ Contam Toxicol       Date:  2006       Impact factor: 7.563

6.  Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus.

Authors:  Tong Zhou; Linlin Du; Yongjian Fan; Yijun Zhou
Journal:  J Virol Methods       Date:  2011-12-30       Impact factor: 2.014

7.  Detection of human Enterovirus 71 reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Authors:  H Yaqing; Z Wenping; Y Zhiyi; W Xionghu; Y Shouyi; Y Hong; D Yingchun; H Guifang
Journal:  Lett Appl Microbiol       Date:  2012-01-18       Impact factor: 2.858

8.  Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.

Authors:  Raja Angamuthu; Subasty Baskaran; Dhinakar Raj Gopal; Jeyanthi Devarajan; Kumanan Kathaperumal
Journal:  J Clin Microbiol       Date:  2011-12-14       Impact factor: 5.948

9.  Description and validation of a novel real-time RT-PCR enterovirus assay.

Authors:  Weston C Hymas; Wade K Aldous; Edward W Taggart; Jeffery B Stevenson; David R Hillyard
Journal:  Clin Chem       Date:  2007-11-26       Impact factor: 8.327

10.  Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases.

Authors:  Minetaro Arita; Hua Ling; Dongmei Yan; Yorihiro Nishimura; Hiromu Yoshida; Takaji Wakita; Hiroyuki Shimizu
Journal:  BMC Infect Dis       Date:  2009-12-16       Impact factor: 3.090
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  5 in total

1.  Evaluation of a viral microarray based on simultaneous extraction and amplification of viral nucleotide acid for detecting human herpesviruses and enteroviruses.

Authors:  Yi Liu; Chunhong Duan; Chunxiu Zhang; Xiaomeng Yang; Yan Zhao; Rui Dong; Jiajing Zhou; Zhongtao Gai
Journal:  PLoS One       Date:  2015-03-16       Impact factor: 3.240

2.  Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique.

Authors:  Tadafumi Yokoyama; Yuko Tasaki; Natsumi Inoue; Naotoshi Sugimoto; Eri Nariai; Sanae Kuramoto; Taizo Wada
Journal:  PLoS One       Date:  2021-11-29       Impact factor: 3.240

Review 3.  The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.

Authors:  Yasaman Sadeghi; Pegah Kananizadeh; Solmaz Ohadian Moghadam; Ahad Alizadeh; Mohammad Reza Pourmand; Neda Mohammadi; Davoud Afshar; Reza Ranjbar
Journal:  Iran J Public Health       Date:  2021-11       Impact factor: 1.429

Review 4.  Recent Developments in Isothermal Amplification Methods for the Detection of Foodborne Viruses.

Authors:  Cassandra Suther; Sloane Stoufer; Yanjiao Zhou; Matthew D Moore
Journal:  Front Microbiol       Date:  2022-03-03       Impact factor: 5.640

5.  Evaluation of a rapid detection for Coxsackievirus B3 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Authors:  A Monazah; M Zeinoddini; A R Saeeidinia
Journal:  J Virol Methods       Date:  2017-04-21       Impact factor: 2.014
  5 in total

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