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Literature DB >> 2442192

Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.

O Chityothin, O Sethabutr, P Echeverria, D N Taylor, U Vongsthongsri, S Tharavanij.

Abstract

Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. Radiolabeled enterotoxin gene RNA transcripts are easier to prepare and avoid the preparation of cloned DNA probes that can be nonspecific if they contain cloning vector DNA.

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Year: 1987 PMID： 2442192 PMCID： PMC269277 DOI： 10.1128/jcm.25.8.1572-1573.1987

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

14 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

3. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

Authors: D A Sack; R B Sack
Journal: Infect Immun Date: 1975-02 Impact factor: 3.441

4. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.

Authors: A G Dean; Y C Ching; R G Williams; L B Harden
Journal: J Infect Dis Date: 1972-04 Impact factor: 5.226

5. Identification by DNA hybridisation of enterotoxigenic Escherichia coli in homes of children with diarrhoea.

Authors: P Echeverria; J Seriwatana; U Leksomboon; C Tirapat; W Chaicumpa; B Rowe
Journal: Lancet Date: 1984-01-14 Impact factor: 79.321

6. Simple and reliable enzyme-linked immunosorbent assay with monoclonal antibodies for detection of Escherichia coli heat-stable enterotoxins.

Authors: M R Thompson; H Brandwein; M LaBine-Racke; R A Giannella
Journal: J Clin Microbiol Date: 1984-07 Impact factor: 5.948

Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

3. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

4. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.

5. Identification by DNA hybridisation of enterotoxigenic Escherichia coli in homes of children with diarrhoea.

6. Simple and reliable enzyme-linked immunosorbent assay with monoclonal antibodies for detection of Escherichia coli heat-stable enterotoxins.

7. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

8. Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

9. Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease.

2. Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.