Literature DB >> 2671025

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.

X Sáez-Llorens1, L M Guzman-Verduzco, S Shelton, J D Nelson, Y M Kupersztoch.   

Abstract

A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2671025      PMCID： PMC267644          DOI： 10.1128/jcm.27.7.1684-1688.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Human diarrheal disease caused by enterotoxigenic Escherichia coli.

Authors:  R B Sack
Journal:  Annu Rev Microbiol       Date:  1975       Impact factor: 15.500

2.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

3.  Cloning, sequencing, and expression in Ficoll-generated minicells of an Escherichia coli heat-stable enterotoxin gene.

Authors:  H Stieglitz; L Cervantes; R Robledo; R Fonseca; L Covarrubias; F Bolivar; Y M Kupersztoch
Journal:  Plasmid       Date:  1988-07       Impact factor: 3.466

4.  Cistrons encoding Escherichia coli heat-labile toxin.

Authors:  W S Dallas; D M Gill; S Falkow
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

5.  The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.

Authors:  H W Smith; C L Gyles
Journal:  J Med Microbiol       Date:  1970-08       Impact factor: 2.472

6.  Linkage of heat-stable enterotoxin activity and ampicillin resistance in a plasmid isolated from an Escherichia coli strain of human origin.

Authors:  H Stieglitz; R Fonseca; J Olarte; Y M Kupersztoch-Portnoy
Journal:  Infect Immun       Date:  1980-11       Impact factor: 3.441

7.  Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

Authors:  D A Sack; R B Sack
Journal:  Infect Immun       Date:  1975-02       Impact factor: 3.441

8.  Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli.

Authors:  D G Evans; D J Evans; N F Pierce
Journal:  Infect Immun       Date:  1973-06       Impact factor: 3.441

9.  Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

Authors:  R H Yolken; H B Greenberg; M H Merson; R B Sack; A Z Kapikian
Journal:  J Clin Microbiol       Date:  1977-11       Impact factor: 5.948

10.  Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors:  R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal:  Infect Immun       Date:  1974-08       Impact factor: 3.441
View more
  1 in total

Review 1.  Some infectious causes of diarrhea in young farm animals.

Authors:  R E Holland
Journal:  Clin Microbiol Rev       Date:  1990-10       Impact factor: 26.132
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.