Literature DB >> 6780599

Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

T Honda, S Taga, Y Takeda, T Miwatani.   

Abstract

The Elek test was modified for detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli. A total of 164 strains of E. coli were tested by the modified Elek test, and the results correlated well with those of the Chinese hamster ovary cell assay and passive immune hemolysis. It is concluded that the modified Elek test is a simple and reproducible assay method for identification of E. coli which produce heat-labile enterotoxin, and is suitable for use in clinical laboratories.

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Year:  1981        PMID： 6780599      PMCID： PMC273710          DOI： 10.1128/jcm.13.1.1-5.1981

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  25 in total

1.  The recognition of toxicogenic bacterial strains in vitro.

Authors:  S D ELEK
Journal:  Br Med J       Date:  1948-03-13

2.  Factors influencing heat-labile Escherichia coli enterotoxin activity.

Authors:  D H Mundell; C R Anselmo; R M Wishnow
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

3.  Neutralization of cholera enterotoxin-induced steroidogenesis by specific antibody.

Authors:  S T Donta
Journal:  J Infect Dis       Date:  1974-03       Impact factor: 5.226

4.  Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.

Authors:  H W Moon; S C Whipp; G W Engstrom; A L Baetz
Journal:  J Infect Dis       Date:  1970-02       Impact factor: 5.226

5.  The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.

Authors:  H W Smith; C L Gyles
Journal:  J Med Microbiol       Date:  1970-08       Impact factor: 2.472

6.  Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Authors:  T Honda; M Shimizu; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1976-10       Impact factor: 3.441

7.  Antigenic difference between polar montrichous and peritrichous flagella of Vibrio parahaemolyticus.

Authors:  S Shinoda; T Honda; Y Takeda; T Miwatani
Journal:  J Bacteriol       Date:  1974-11       Impact factor: 3.490

8.  Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

Authors:  D A Sack; R B Sack
Journal:  Infect Immun       Date:  1975-02       Impact factor: 3.441

9.  Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis.

Authors:  M Levner; F P Wiener; B A Rubin
Journal:  Infect Immun       Date:  1977-01       Impact factor: 3.441

10.  Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors:  R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal:  Infect Immun       Date:  1974-08       Impact factor: 3.441
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  46 in total

1.  Comparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin.

Authors:  B Cryan
Journal:  J Clin Microbiol       Date:  1990-04       Impact factor: 5.948

Review 2.  Escherichia coli that cause diarrhea.

Authors:  M M Levine; P Vial
Journal:  Indian J Pediatr       Date:  1988 Mar-Apr       Impact factor: 1.967

3.  Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.

Authors:  O Chityothin; O Sethabutr; P Echeverria; D N Taylor; U Vongsthongsri; S Tharavanij
Journal:  J Clin Microbiol       Date:  1987-08       Impact factor: 5.948

Review 4.  Some infectious causes of diarrhea in young farm animals.

Authors:  R E Holland
Journal:  Clin Microbiol Rev       Date:  1990-10       Impact factor: 26.132

5.  Some observations on the Biken test for the detection of enterotoxigenic Escherichia coli.

Authors:  Y Takeda; T Honda; H Sima; I Hernandez; A Chantakit; T Takeda; T Miwatani
Journal:  Eur J Clin Microbiol       Date:  1983-02       Impact factor: 3.267

6.  Escherichia coli associated with childhood diarrheas.

Authors:  W C Yam; M L Lung; C Y Yeung; J S Tam; M H Ng
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

7.  Lincomycin increases the half-life of beta-lactamase mRNA.

Authors:  O Matsushita; A Okabe; H Hayashi; Y Kanemasa
Journal:  Antimicrob Agents Chemother       Date:  1989-06       Impact factor: 5.191

8.  Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.

Authors:  T Ito; S Kuwahara; T Yokota
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

9.  Three-year prospective study of intestinal pathogens in Madrid, Spain.

Authors:  A C Velasco; M L Mateos; G Más; A Pedraza; M Díez; A Gutiérrez
Journal:  J Clin Microbiol       Date:  1984-08       Impact factor: 5.948

10.  Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment.

Authors:  J P Craig; K Yamamoto; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1981-10       Impact factor: 3.441
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