Literature DB >> 24373999

High-performance liquid chromatography purification of chemically modified RNA aptamers.

Chi-Yen Lin1, Zhen Huang1, William Jaremko1, Li Niu2.   

Abstract

2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Chemically modified RNA; HPLC; RNA purification and separation

Mesh：

Substances：

Year:  2013        PMID： 24373999      PMCID： PMC5577503          DOI： 10.1016/j.ab.2013.12.022

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  24 in total

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Authors:  A M Pyle; F L Murphy; T R Cech
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9.  The RNA Modification Database, RNAMDB: 2011 update.

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Review 10.  Aptamers as therapeutics.

Authors:  Anthony D Keefe; Supriya Pai; Andrew Ellington
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Review 1.  Recent Methods for Purification and Structure Determination of Oligonucleotides.

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2.  HPLC methods for purity evaluation of man-made single-stranded RNAs.

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