Literature DB >> 2437323

cDNA cloning with a retrovirus expression vector: generation of a pp60c-src cDNA clone.

P L Kaplan, S Simon, C A Cartwright, W Eckhart.   

Abstract

We used a murine retroviral expression vector, containing a genomic clone of the chicken c-src gene, a bacterial origin of replication, and a selectable marker, to remove 10 introns from the c-src gene. All 10 introns were removed accurately, and no mutations were introduced. The processed gene encoded a functional pp60c-src protein tyrosine kinase.

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Year:  1987        PMID： 2437323      PMCID： PMC254166     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  29 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.

Authors:  R A Laskey; A D Mills
Journal:  FEBS Lett       Date:  1977-10-15       Impact factor: 4.124

4.  Characterization of Rous sarcoma virus src gene products synthesized in vitro.

Authors:  K Beemon; T Hunter
Journal:  J Virol       Date:  1978-11       Impact factor: 5.103

5.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

6.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

7.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

8.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Retrovirus shuttle vector for study of kinase activities of pp60c-src synthesized in vitro and overproduced in vivo.

Authors:  H Piwnica-Worms; D R Kaplan; M Whitman; T M Roberts
Journal:  Mol Cell Biol       Date:  1986-06       Impact factor: 4.272
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  9 in total

1.  Functional asymmetry of the regions juxtaposed to the membrane-binding sequence of polyomavirus middle T antigen.

Authors:  J Dahl; U Thathamangalam; R Freund; T L Benjamin
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

2.  A retroviral promoter is sufficient to convert proto-src to a transforming gene that is distinct from the src gene of Rous sarcoma virus.

Authors:  H Zhou; P H Duesberg
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

3.  Analysis of cDNAs of the proto-oncogene c-src: heterogeneity in 5' exons and possible mechanism for the genesis of the 3' end of v-src.

Authors:  T Dorai; J B Levy; L Kang; J S Brugge; L H Wang
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

4.  Mutation of the serine 312 phosphorylation site does not alter the ability of mouse p53 to inhibit simian virus 40 DNA replication in vivo.

Authors:  D W Meek; W Eckhart
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

5.  Mutation of a cysteine residue in polyomavirus middle T antigen abolishes interactions with protein phosphatase 2A, pp60c-src, and phosphatidylinositol-3 kinase, activation of c-fos expression, and cellular transformation.

Authors:  G M Glenn; W Eckhart
Journal:  J Virol       Date:  1993-04       Impact factor: 5.103

6.  Phenotypic correction of factor IX deficiency in skin fibroblasts of hemophilic dogs.

Authors:  J H Axelrod; M S Read; K M Brinkhous; I M Verma
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

7.  Activated lck tyrosine protein kinase stimulates antigen-independent interleukin-2 production in T cells.

Authors:  K Luo; B M Sefton
Journal:  Mol Cell Biol       Date:  1992-10       Impact factor: 4.272

8.  Amino-terminal regions of polyomavirus middle T antigen are required for interactions with protein phosphatase 2A.

Authors:  G M Glenn; W Eckhart
Journal:  J Virol       Date:  1995-06       Impact factor: 5.103

9.  Mutation of a site of tyrosine phosphorylation in the lymphocyte-specific tyrosine protein kinase, p56lck, reveals its oncogenic potential in fibroblasts.

Authors:  K E Amrein; B M Sefton
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205
  9 in total

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