Literature DB >> 2436041

Human ribosomal DNA fragments amplified in hamster cells are transcribed only by RNA polymerase II and are not silver stained.

V N Dhar, D A Miller, A B Kulkarni, O J Miller.   

Abstract

Cloned human rRNA gene fragments that included the promoter region were introduced into Chinese hamster dihydrofolate reductase-deficient (dhfr-) cells by cotransformation with a dhfr minigene and amplified by selection for methotrexate resistance. The human ribosomal DNA was transcribed by RNA polymerase II, not RNA polymerase I or III. The metaphase chromosome regions containing the transcriptionally active human ribosomal DNA failed to show silver staining.

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Year:  1987        PMID： 2436041      PMCID： PMC365206          DOI： 10.1128/mcb.7.3.1289-1292.1987

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  24 in total

1.  Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters.

Authors:  S T Smale; R Tjian
Journal:  Mol Cell Biol       Date:  1985-02       Impact factor: 4.272

2.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

3.  Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.

Authors:  M E Greenberg; E B Ziff
Journal:  Nature       Date:  1984 Oct 4-10       Impact factor: 49.962

4.  The absence of a human-specific ribosomal DNA transcription factor leads to nucleolar dominance in mouse greater than human hybrid cells.

Authors:  R Miesfeld; B Sollner-Webb; C Croce; N Arnheim
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

5.  A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I.

Authors:  V Cizewski; B Sollner-Webb
Journal:  Nucleic Acids Res       Date:  1983-10-25       Impact factor: 16.971

6.  Ribosomal RNA transcription in vitro is species specific.

Authors:  I Grummt; E Roth; M R Paule
Journal:  Nature       Date:  1982-03-11       Impact factor: 49.962

7.  Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.

Authors:  G S McKnight; R D Palmiter
Journal:  J Biol Chem       Date:  1979-09-25       Impact factor: 5.157

8.  On the mechanism of nucleolar dominance in mouse-human somatic cell hybrids.

Authors:  T Onishi; C Berglund; R H Reeder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

9.  Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.

Authors:  C S Gasser; C C Simonsen; J W Schilling; R T Schimke
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

10.  Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

Authors:  G Urlaub; L A Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205
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  4 in total

1.  RNA polymerase switch in transcription of yeast rDNA: role of transcription factor UAF (upstream activation factor) in silencing rDNA transcription by RNA polymerase II.

Authors:  L Vu; I Siddiqi; B S Lee; C A Josaitis; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-13       Impact factor: 11.205

2.  Transcription of chromosomal rRNA genes by both RNA polymerase I and II in yeast uaf30 mutants lacking the 30 kDa subunit of transcription factor UAF.

Authors:  I N Siddiqi; J A Dodd; L Vu; K Eliason; M L Oakes; J Keener; R Moore; M K Young; M Nomura
Journal:  EMBO J       Date:  2001-08-15       Impact factor: 11.598

3.  A polymerase switch in the synthesis of rRNA in Saccharomyces cerevisiae.

Authors:  H Conrad-Webb; R A Butow
Journal:  Mol Cell Biol       Date:  1995-05       Impact factor: 4.272

4.  HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation.

Authors:  Arjuna Rao Vallabhaneni; Merita Kabashi; Matt Haymowicz; Kushal Bhatt; Violet Wayman; Shazia Ahmed; Heather Conrad-Webb
Journal:  Curr Genet       Date:  2021-08-06       Impact factor: 3.886
  4 in total

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