Literature DB >> 6959133

Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.

C S Gasser, C C Simonsen, J W Schilling, R T Schimke.   

Abstract

We have constructed a number of abbreviated dihydrofolate reductase (DHFR) genes by using cloned mouse genomic and cDNA sequences. These genes contain 1.0 kilobase of 5' flanking genomic sequence and varying portions of the 3' non-coding region. Two of the genes contain the first two introns of the DHFR gene; the other three lack introns. Transfection of DHFR-deficient Chinese hamster ovary cells with any of these constructed genes results in cells with the DHFR+ phenotype. Treatment of the transfectants with methotrexate, a folate antagonist, leads to the emergence of methotrexate-resistant colonies which have amplified the transfected genes.

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Year:  1982        PMID： 6959133      PMCID： PMC347159          DOI： 10.1073/pnas.79.21.6522

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Splicing and the formation of stable RNA.

Authors:  D H Hamer; P Leder
Journal:  Cell       Date:  1979-12       Impact factor: 41.582

2.  Deletion mutants of simian virus 40 defective in biosynthesis of late viral mRNA.

Authors:  C J Lai; G Khoury
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

3.  Species identification and genome mapping of cytoplasmic adenovirus type 2 RNAs synthesized late in infection.

Authors:  M McGrogan; H J Raskas
Journal:  J Virol       Date:  1977-08       Impact factor: 5.103

4.  Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

Authors:  A C Chang; J H Nunberg; R J Kaufman; H A Erlich; R T Schimke; S N Cohen
Journal:  Nature       Date:  1978-10-19       Impact factor: 49.962

5.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

6.  Rescue of a splicing defective mutant by insertion of an heterologous intron.

Authors:  P Gruss; G Khoury
Journal:  Nature       Date:  1980-08-07       Impact factor: 49.962

7.  Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors:  F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157

8.  Structure and genomic organization of the mouse dihydrofolate reductase gene.

Authors:  J H Nunberg; R J Kaufman; A C Chang; S N Cohen; R T Schimke
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

9.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

10.  Regulation of dihydrofolate reductase synthesis in an overproducing 3T6 cell line during transition from resting to growing state.

Authors:  L M Wiedemann; L F Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205
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  49 in total

1.  Splicing is required for rapid and efficient mRNA export in metazoans.

Authors:  M J Luo; R Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

2.  Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.

Authors:  Y Huang; K M Wimler; G G Carmichael
Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

3.  Simian virus 40 late transcripts lacking excisable intervening sequences are defective in both stability in the nucleus and transport to the cytoplasm.

Authors:  W S Ryu; J E Mertz
Journal:  J Virol       Date:  1989-10       Impact factor: 5.103

4.  Polymorphism rs2239185 in vitamin D receptor gene is associated with severe community-acquired pneumonia of children in Chinese Han population: a case-control study.

Authors:  Wei Li; Linying Guo; Hongri Li; Chunrong Sun; Xiaodai Cui; Guowei Song; Jianhua Wang; Qi Zhang
Journal:  Eur J Pediatr       Date:  2014-11-01       Impact factor: 3.183

5.  Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA.

Authors:  M T Huang; C M Gorman
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

6.  Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells.

Authors:  R S McIvor; C C Simonsen
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

7.  Splice site requirement for the efficient accumulation of polyoma virus late mRNAs.

Authors:  N L Barrett; G G Carmichael; Y Luo
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971

8.  Similarities between human ataxia fibroblasts and murine SCID cells: high sensitivity to gamma rays and high frequency of methotrexate-induced DHFR gene amplification, but normal radiosensitivity to densely ionizing alpha particles.

Authors:  C Lücke-Huhle
Journal:  Radiat Environ Biophys       Date:  1994       Impact factor: 1.925

9.  Isolation and expression of an altered mouse dihydrofolate reductase cDNA.

Authors:  C C Simonsen; A D Levinson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

10.  Influence of chromosomal integration on glucocorticoid-regulated transcription of growth-stimulating papillomavirus genes E6 and E7 in cervical carcinoma cells.

Authors:  M von Knebel Doeberitz; T Bauknecht; D Bartsch; H zur Hausen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205
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