Literature DB >> 2434426

Characterization of Plasmodium yoelii monoclonal antibodies directed against stage-specific sporozoite antigens.

Y Charoenvit, M F Leef, L F Yuan, M Sedegah, R L Beaudoin.   

Abstract

A battery of monoclonal antibodies against Plasmodium yoelii sporozoites was produced. Five of these (NYS1 through NYS5) were selected for characterization. All five were positive in the indirect immunofluorescent antibody test with P. yoelii sporozoites; however, each showed a different immunofluorescence pattern. Although NYS1 (immunoglobulin G3 [IgG3]), NYS2 (IgG3), and NYS3 (IgM) were positive in the circumsporozoite precipitation test, only NYS1 and NYS2 were able to neutralize sporozoite infectivity in mice. NYS4 (IgM) and NYS5 (IgG1) were not positive in the precipitation test and did not protect mice from sporozoite infection. All except NYS4 were species as well as stage specific. NYS4 cross-reacted with sporozoites of P. berghei. Electrophoretic immunoblotting analysis showed that these monoclonal antibodies detected sporozoite antigens of various molecular weights. Inhibition enzyme-linked immunosorbent assays indicated that each recognized a different antigenic epitope. The differences in their immunochemical and biological reactivity make them useful for screening a variety of P. yoelii antigens in recombinant DNA libraries. These antigens will be used in an animal model system for vaccine development.

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Year:  1987        PMID： 2434426      PMCID： PMC260381          DOI： 10.1128/iai.55.3.604-608.1987

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  19 in total

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Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
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2.  Diffusion-in-gel methods for immunological analysis.

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Journal:  Prog Allergy       Date:  1958

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

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4.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

5.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

6.  Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite.

Authors:  N Yoshida; R S Nussenzweig; P Potocnjak; V Nussenzweig; M Aikawa
Journal:  Science       Date:  1980-01-04       Impact factor: 47.728

7.  Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. V. In vitro effects of immune serum on sporozoites.

Authors:  J Vanderberg; R Nussenzweig; H Most
Journal:  Mil Med       Date:  1969-09       Impact factor: 1.437

8.  Rapid, large-scale isolation of Plasmodium berghei sporozoites from infected mosquitoes.

Authors:  N D Pacheco; C P Strome; F Mitchell; M P Bawden; R L Beaudoin
Journal:  J Parasitol       Date:  1979-06       Impact factor: 1.276

9.  Sodium dodecyl sulfate in protein chemistry.

Authors:  T V Waehneldt
Journal:  Biosystems       Date:  1975-03       Impact factor: 1.973

10.  Monovalent fragments (Fab) of monoclonal antibodies to a sporozoite surface antigen (Pb44) protect mice against malarial infection.

Authors:  P Potocnjak; N Yoshida; R S Nussenzweig; V Nussenzweig
Journal:  J Exp Med       Date:  1980-06-01       Impact factor: 14.307
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  36 in total

1.  The binding of the circumsporozoite protein to cell surface heparan sulfate proteoglycans is required for plasmodium sporozoite attachment to target cells.

Authors:  C Pinzon-Ortiz; J Friedman; J Esko; P Sinnis
Journal:  J Biol Chem       Date:  2001-05-14       Impact factor: 5.157

2.  Immunogenicity of well-characterized synthetic Plasmodium falciparum multiple antigen peptide conjugates.

Authors:  M B Joshi; A A Gam; R A Boykins; S Kumar; J Sacci; S L Hoffman; H L Nakhasi; R T Kenney
Journal:  Infect Immun       Date:  2001-08       Impact factor: 3.441

3.  Simultaneous induction of multiple antigen-specific cytotoxic T lymphocytes in nonhuman primates by immunization with a mixture of four Plasmodium falciparum DNA plasmids.

Authors:  R Wang; D L Doolan; Y Charoenvit; R C Hedstrom; M J Gardner; P Hobart; J Tine; M Sedegah; V Fallarme; J B Sacci; M Kaur; D M Klinman; S L Hoffman; W R Weiss
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

Review 4.  The development of a multivalent DNA vaccine for malaria.

Authors:  R C Hedstrom; D L Doolan; R Wang; M J Gardner; A Kumar; M Sedegah; R A Gramzinski; J B Sacci; Y Charoenvit; W R Weiss; M Margalith; J A Norman; P Hobart; S L Hoffman
Journal:  Springer Semin Immunopathol       Date:  1997

5.  CD8+ T cells (cytotoxic/suppressors) are required for protection in mice immunized with malaria sporozoites.

Authors:  W R Weiss; M Sedegah; R L Beaudoin; L H Miller; M F Good
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

6.  CD4(+) T-cell- and gamma interferon-dependent protection against murine malaria by immunization with linear synthetic peptides from a Plasmodium yoelii 17-kilodalton hepatocyte erythrocyte protein.

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Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

7.  The relative contribution of antibodies, CD4+ and CD8+ T cells to sporozoite-induced protection against malaria.

Authors:  M Rodrigues; R S Nussenzweig; F Zavala
Journal:  Immunology       Date:  1993-09       Impact factor: 7.397

8.  Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies.

Authors:  George Jiang; Meng Shi; Solomon Conteh; Nancy Richie; Glenna Banania; Harini Geneshan; Anais Valencia; Priti Singh; Joao Aguiar; Keith Limbach; Kurt I Kamrud; Jonathan Rayner; Jonathan Smith; Joseph T Bruder; C Richter King; Takafumi Tsuboi; Satoru Takeo; Yaeta Endo; Denise L Doolan; Thomas L Richie; Walter R Weiss
Journal:  PLoS One       Date:  2009-08-10       Impact factor: 3.240

9.  Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicity.

Authors:  David P Regis; Carlota Dobaño; Paola Quiñones-Olson; Xiaowu Liang; Norma L Graber; Maureen E Stefaniak; Joseph J Campo; Daniel J Carucci; David A Roth; Huaping He; Philip L Felgner; Denise L Doolan
Journal:  Mol Biochem Parasitol       Date:  2007-11-22       Impact factor: 1.759

10.  Characterization of Plasmodium falciparum sporozoite surface protein 2.

Authors:  W O Rogers; A Malik; S Mellouk; K Nakamura; M D Rogers; A Szarfman; D M Gordon; A K Nussler; M Aikawa; S L Hoffman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-01       Impact factor: 11.205
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