| Literature DB >> 1120181 |
Abstract
This review summarizes in a brief manner the main aspects of the application of sodium dodecyl sulfate (SDS) to protein chemistry. The principal problems of SDS-polyacrylamide gel electrophoresis are described, as well as the anomalous behavior of protein-SDS complexes and the inactivation of enzymes due to variable binding of SDS to the polypeptides studied. The particular value of SDS in elucidating the protein composition of biological membranes and in membrane-reconstitution experiments is discussed.Entities:
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Year: 1975 PMID： 1120181 DOI： 10.1016/0303-2647(75)90025-8
Source DB: PubMed Journal: Biosystems ISSN： 0303-2647 Impact factor: 1.973