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Literature DB >> 113458

A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

J F Kearney, A Radbruch, B Liesegang, K Rajewsky.

Abstract

We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.

Entities: CellLine Disease Species

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Year: 1979 PMID： 113458

Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422

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Cited

466 in total

1. Monoclonal antibodies against the minimal DNA-binding domain in the carboxyl-terminal region of human immunodeficiency virus type 1 integrase.

Authors: T Ishikawa; N Okui; N Kobayashi; R Sakuma; T Kitamura; Y Kitamura
Journal: J Virol Date: 1999-05 Impact factor: 5.103

2. B-cell repertoire specific for an unfolded self-determinant of mouse lysozyme escape tolerance and dominantly participate in the autoantibody response.

Authors: Yoshiyuki Tsujihata; Yousuke Mizukami; Tadashi Ueda; Takanori So; Taiji Imoto
Journal: Immunology Date: 2002-12 Impact factor: 7.397

3. Regulation of heme biosynthesis in Salmonella typhimurium: activity of glutamyl-tRNA reductase (HemA) is greatly elevated during heme limitation by a mechanism which increases abundance of the protein.

Authors: L Y Wang; L Brown; M Elliott; T Elliott
Journal: J Bacteriol Date: 1997-05 Impact factor: 3.490

4. Residues 20, 22, and 26 determine the subtype specificities of staphylococcal enterotoxins C1 and C2.

Authors: T N Turner; C L Smith; G A Bohach
Journal: Infect Immun Date: 1992-02 Impact factor: 3.441

Review 5. Monoclonal antibodies to epidermal growth factor receptors in studies of receptor structure and function.

Authors: T Kawamoto; G H Sato; K Takahashi; M Nishi; S Taniguchi; J D Sato
Journal: Cytotechnology Date: 1990-05 Impact factor: 2.058

6. Silent and expressed sister Mup genes are located within distinct chromatin domains: analysis by pulsed-field gel electrophoresis and polymerase chain reaction-supplemented DNase I digestion.

Authors: M Rodriguez; E Derman
Journal: Mol Cell Biol Date: 1992-03 Impact factor: 4.272

A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

1. Monoclonal antibodies against the minimal DNA-binding domain in the carboxyl-terminal region of human immunodeficiency virus type 1 integrase.

2. B-cell repertoire specific for an unfolded self-determinant of mouse lysozyme escape tolerance and dominantly participate in the autoantibody response.

3. Regulation of heme biosynthesis in Salmonella typhimurium: activity of glutamyl-tRNA reductase (HemA) is greatly elevated during heme limitation by a mechanism which increases abundance of the protein.

4. Residues 20, 22, and 26 determine the subtype specificities of staphylococcal enterotoxins C1 and C2.

Review 5. Monoclonal antibodies to epidermal growth factor receptors in studies of receptor structure and function.

6. Silent and expressed sister Mup genes are located within distinct chromatin domains: analysis by pulsed-field gel electrophoresis and polymerase chain reaction-supplemented DNase I digestion.

7. Monkey-derived monoclonal antibodies against Plasmodium falciparum.

8. Monoclonal antibody detection of IncF group plasmid-encoded TraT protein in clinical isolates of Escherichia coli.

9. Studies of mammalian ornithine decarboxylase using a monoclonal antibody.

10. Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.