| Literature DB >> 24333002 |
Adam C Rea1, Laura N Vandenberg2, Rebecca E Ball3, Ashley A Snouffer4, Alicia G Hudson3, Yue Zhu1, Duncan E McLain5, Lindsey L Johnston4, James D Lauderdale3, Michael Levin2, Timothy M Dore6.
Abstract
Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.Entities:
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Year: 2013 PMID： 24333002 PMCID： PMC3909739 DOI： 10.1016/j.chembiol.2013.11.005
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521