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Literature DB >> 24257710

Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.

Abstract

Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparatively high concentrations. They developed to mature embryos when the auxin was reduced or omitted.

Entities: Species

Year: 1982 PMID： 24257710 DOI： 10.1007/BF00270237

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

4 in total

1. Culture and plant regeneration of Hyoscyamus protoplasts.

Authors: H Lörz; W Wernicke; I Potrykus
Journal: Planta Med Date: 1979-05 Impact factor: 3.352

2. Isolation and culture of cereal protoplasts : Part 2: Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum.

Authors: V Vasil; I K Vasil
Journal: Theor Appl Genet Date: 1980-05 Impact factor: 5.699

3. Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts.

Authors: F J Zapata; K C Sink
Journal: Theor Appl Genet Date: 1981-09 Impact factor: 5.699

4. Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species.

Authors: A Vardi; P Spiegel-Roy; E Galun
Journal: Theor Appl Genet Date: 1982-06 Impact factor: 5.699

4 in total

16 in total

1. Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) Czern.

Authors: G Chatterjee; S R Sikdar; S Das; S K Sen
Journal: Plant Cell Rep Date: 1985-10 Impact factor: 4.570

2. A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus.

Authors: T L Barsby; S A Yarrow; J F Shepard
Journal: Plant Cell Rep Date: 1986-04 Impact factor: 4.570

3. Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

Authors: S R Sikdar; G Chatterjee; S Das; S K Sen
Journal: Plant Cell Rep Date: 1987-12 Impact factor: 4.570

4. Effect of various irradiation treatments of plant protoplasts on the transformation rates after direct gene transfer.

Authors: F Köhler; I Benediktsson; G Cardon; C S Andreo; O Schieder
Journal: Theor Appl Genet Date: 1990-05 Impact factor: 5.699

5. A novel method for regenerating plants from mesophyll protoplasts of Arabidopsis thaliana line WS.

Authors: S Sando; N Goto
Journal: Plant Cell Rep Date: 1994-12 Impact factor: 4.570

6. Plant regeneration from mesophyll-protoplasts of four different ecotypes and two marker lines from Arabidopsis thaliana using a unique protocol.

Authors: J Siemens; M Torres; M Morgner; M D Sacristán
Journal: Plant Cell Rep Date: 1993-08 Impact factor: 4.570

7. Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line.

Authors: C Golz; F Köhler; O Schieder
Journal: Plant Mol Biol Date: 1990-09 Impact factor: 4.076

8. A simple culture method for Brassica hypototyl protoplasts.

Authors: P V Chuong; K P Pauls; W D Beversdorf
Journal: Plant Cell Rep Date: 1985-02 Impact factor: 4.570

9. Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.

Authors: J Paszkowski; B Pisan; R D Shillito; T Hohn; B Hohn; I Potrykus
Journal: Plant Mol Biol Date: 1986-09 Impact factor: 4.076

10. Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion.

Authors: M D Sacristán; M Gerdemann-Knörck; O Schieder
Journal: Theor Appl Genet Date: 1989-08 Impact factor: 5.699