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Literature DB >> 1320426

Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.

Abstract

The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.

Entities: Chemical Species

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Year: 1992 PMID： 1320426 DOI： 10.1007/bf00023396

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

20 in total

1. Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.

Authors: R Töpfer; M Pröls; J Schell; H H Steinbiß
Journal: Plant Cell Rep Date: 1988-06 Impact factor: 4.570

2. A simple and rapid method for the preparation of total plant DNA.

Authors: H Junghans; M Metzlaff
Journal: Biotechniques Date: 1990-02 Impact factor: 1.993

3. Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation.

Authors: S E Stachel; P C Zambryski
Journal: Cell Date: 1986-10-24 Impact factor: 41.582

4. The isolation, culture and regeneration of Petunia leaf protoplasts.

Authors: E M Frearson; J B Power; E C Cocking
Journal: Dev Biol Date: 1973-07 Impact factor: 3.582

5. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.

Authors: A Depicker; M De Wilde; G De Vos; R De Vos; M Van Montagu; J Schell
Journal: Plasmid Date: 1980-03 Impact factor: 3.466

2. Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata).

Authors: T D Metz; R Dixit; E D Earle
Journal: Plant Cell Rep Date: 1995-12 Impact factor: 4.570

2 in total

Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.

1. Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.

2. A simple and rapid method for the preparation of total plant DNA.

3. Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation.

4. The isolation, culture and regeneration of Petunia leaf protoplasts.

5. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.

6. Brassica grown gall tumourigenesis and in vitro of transformed tissue.

7. Transgenic plants as tools to study the molecular organization of plant genes.

8. Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium.

9. Transformation and regeneration of Brassica oleracea mediated by an oncogenic Agrobacterium tumefaciens.

10. New cloning vehicles for transformation of higher plants.

1. Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.

2. Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata).