Literature DB >> 24301191

Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.

S N Sheikholeslam1, D P Weeks.   

Abstract

High frequency transformation of Arabidopsis thaliana leaf explants has been obtained using a disarmed Ti plasmid containing the coding region of a neomycin phosphotransferase gene (NPT II) as a selectable marker. The rate of transformation ranged from 55 to 63 percent when acetosyringone (AS), a natural wound response molecule, was added to an Agrobacterium tumefaciens culture prior to incubation with leaf segments. Without acetosyringone, the transformation rate was approximately 2 to 3 percent. Calli resistant to G418 were regenerated into mature flowering plants in the presence of 10 μg/ml G418. Southern analysis and neomycin phosphotransferase assays confirmed the insertion and expression of the NPT II gene in regenerated Arabidopsis plants.

Entities:  

Year:  1987        PMID： 24301191     DOI： 10.1007/BF00021308

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  13 in total

1.  A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. 1983.

Authors:  M W Bevan; R B Flavell; M D Chilton
Journal:  Biotechnology       Date:  1992

2.  Streptomycin-resistant plants from callus culture of haploid tobacco.

Authors:  P Maliga; A Sz-Breznovits; L Márton
Journal:  Nat New Biol       Date:  1973-07-04

3.  Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from agrobacterium to the plant genome.

Authors:  K Wang; L Herrera-Estrella; M Van Montagu; P Zambryski
Journal:  Cell       Date:  1984-09       Impact factor: 41.582

4.  Expression of bacterial genes in plant cells.

Authors:  R T Fraley; S G Rogers; R B Horsch; P R Sanders; J S Flick; S P Adams; M L Bittner; L A Brand; C L Fink; J S Fry; G R Galluppi; S B Goldberg; N L Hoffmann; S C Woo
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  A plant cell factor induces Agrobacterium tumefaciens vir gene expression.

Authors:  S E Stachel; E W Nester; P C Zambryski
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

6.  Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System.

Authors:  G An; B D Watson; C C Chiang
Journal:  Plant Physiol       Date:  1986-05       Impact factor: 8.340

7.  Characterization of the genome of Arabidopsis thaliana.

Authors:  R E Pruitt; E M Meyerowitz
Journal:  J Mol Biol       Date:  1986-01-20       Impact factor: 5.469

8.  A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources.

Authors:  D P Weeks; N Beerman; O M Griffith
Journal:  Anal Biochem       Date:  1986-02-01       Impact factor: 3.365

9.  Chimeric genes as dominant selectable markers in plant cells.

Authors:  L Herrera-Estrella; M D Block; E Messens; J P Hernalsteens; M V Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

Authors:  P Zambryski; H Joos; C Genetello; J Leemans; M V Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  44 in total

Review 1.  Agrobacterium and plant genetic engineering.

Authors:  P J Hooykaas; R A Schilperoort
Journal:  Plant Mol Biol       Date:  1992-05       Impact factor: 4.076

2.  Genetic transformation of Arabidopsis thaliana zygotic embryos and identification of critical parameters influencing transformation efficiency.

Authors:  R S Sangwan; Y Bourgeois; B S Sangwan-Norreel
Journal:  Mol Gen Genet       Date:  1991-12

3.  Facile transformation of Arabidopsis.

Authors:  L Márton; J Browse
Journal:  Plant Cell Rep       Date:  1991-08       Impact factor: 4.570

4.  Direct gene transfer to protoplasts of Arabidopsis thaliana.

Authors:  H Karesch; R Bilang; O Mittelsten Scheid; I Potrykus
Journal:  Plant Cell Rep       Date:  1991-02       Impact factor: 4.570

5.  Factors influencing T-DNA transfer in Agrobacterium-mediated transformation of sugarbeet.

Authors:  B Jacq; O Lesobre; R S Sangwan; B S Sangwan-Norreel
Journal:  Plant Cell Rep       Date:  1993-09       Impact factor: 4.570

6.  Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple.

Authors:  D J James; S Uratsu; J Cheng; P Negri; P Viss; A M Dandekar
Journal:  Plant Cell Rep       Date:  1993-08       Impact factor: 4.570

7.  Factors influencing the efficiency of T-DNA transfer during co-cultivation of Antirrhinum majus with Agrobacterium tumefaciens.

Authors:  P Holford; N Hernandez; H J Newbury
Journal:  Plant Cell Rep       Date:  1992-05       Impact factor: 4.570

8.  High-efficiency Agrobacterium rhizogenes-mediated transformation of heat inducible sHSP18.2-GUS in Nicotiana tabacum.

Authors:  Shih-Cheng Chen; Hui-Wen Liu; Kung-Ta Lee; Takashi Yamakawa
Journal:  Plant Cell Rep       Date:  2006-07-28       Impact factor: 4.570

9.  The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

Authors:  I Godwin; G Todd; B Ford-Lloyd; H J Newbury
Journal:  Plant Cell Rep       Date:  1991-04       Impact factor: 4.570

10.  Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants.

Authors:  Amjad Masood Husaini
Journal:  Plant Cell Rep       Date:  2009-12-03       Impact factor: 4.570
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