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Literature DB >> 24220714

Direct gene transfer to protoplasts of Arabidopsis thaliana.

H Karesch¹, R Bilang, O Mittelsten Scheid, I Potrykus.

Abstract

We performed a series of direct gene transfer experiments with protoplasts of Arabidopsis thaliana ecotype Zürich. An average of more than 100 transformants were selected per 10(6)6 treated protoplasts. Stable transformation was confirmed by integration of the marker gene into high molecular weight DNA and by its genetic transmission to subsequent offspring generations.

Entities: Species

Year: 1991 PMID： 24220714 DOI： 10.1007/BF00232334

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

13 in total

Review 1. Arabidopsis, a useful weed.

Authors: E M Meyerowitz
Journal: Cell Date: 1989-01-27 Impact factor: 41.582

2. Rapid isolation of high molecular weight plant DNA.

Authors: M G Murray; W F Thompson
Journal: Nucleic Acids Res Date: 1980-10-10 Impact factor: 16.971

3. Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System.

Authors: G An; B D Watson; C C Chiang
Journal: Plant Physiol Date: 1986-05 Impact factor: 8.340

4. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors: B Damm; R Schmidt; L Willmitzer
Journal: Mol Gen Genet Date: 1989-05

5. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

Authors: A M Lloyd; A R Barnason; S G Rogers; M C Byrne; R T Fraley; R B Horsch
Journal: Science Date: 1986-10-24 Impact factor: 47.728

6. A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis.

Authors: K A Feldmann; M D Marks; M L Christianson; R S Quatrano
Journal: Science Date: 1989-03-10 Impact factor: 47.728

7. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.

Authors: L Gritz; J Davies
Journal: Gene Date: 1983-11 Impact factor: 3.688

8. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

9. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.

Authors: S N Sheikholeslam; D P Weeks
Journal: Plant Mol Biol Date: 1987-07 Impact factor: 4.076

10. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

Authors: R Schmidt; L Willmitzer
Journal: Plant Cell Rep Date: 1988-12 Impact factor: 4.570

8 in total

1. Reversible inactivation of a transgene in Arabidopsis thaliana.

Authors: O Mittelsten Scheid; J Paszkowski; I Potrykus
Journal: Mol Gen Genet Date: 1991-08

2. Transformation of haploid Datura innoxia protoplasts and analysis of the plasmid integration pattern in regenerated transgenic plants.

Authors: T Schmidt-Rogge; M Meixner; V Srivastava; S Guha-Mukherjee; O Schieder
Journal: Plant Cell Rep Date: 1993-05 Impact factor: 4.570

Direct gene transfer to protoplasts of Arabidopsis thaliana.

Review 1. Arabidopsis, a useful weed.

2. Rapid isolation of high molecular weight plant DNA.

3. Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System.

4. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

5. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

6. A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis.

7. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.

8. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

9. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.

10. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

1. Reversible inactivation of a transgene in Arabidopsis thaliana.

2. Transformation of haploid Datura innoxia protoplasts and analysis of the plasmid integration pattern in regenerated transgenic plants.

3. A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts.

4. Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement.

5. Gene inactivation in Arabidopsis thaliana is not accompanied by an accumulation of repeat-induced point mutations.

6. Gene targeting in Arabidopsis thaliana.

7. Genetic rearrangements can modify chromatin features at epialleles.

8. Polyploidy-associated paramutation in Arabidopsis is determined by small RNAs, temperature, and allele structure.