Literature DB >> 24240423

High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

R Schmidt1, L Willmitzer.   

Abstract

A highly efficient and fast Agrobacterium-mediated leaf disc transformation system for the Arabidopsis thaliana L. genotype C24 was developed. This protocol is also amenable to other ecotypes - as could be shown for Landsberg erecta and Wassllewskija. Besides the hygromycin selection also the G418 and kanamycin selection were established. Furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as explant source for transformation experiments.

Entities:  

Year:  1988        PMID： 24240423     DOI： 10.1007/BF00272763

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  19 in total

1.  High-frequency plant regeneration from cultured cotyledons of Arabidopsis thaliana.

Authors:  D A Patton; D W Meinke
Journal:  Plant Cell Rep       Date:  1988-06       Impact factor: 4.570

2.  A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Authors:  L A Otten; R A Schilperoort
Journal:  Biochim Biophys Acta       Date:  1978-12-08

3.  A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors:  B Reiss; R Sprengel; H Will; H Schaller
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

4.  The role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor.

Authors:  A G Hepburn; L E Clarke; L Pearson; J White
Journal:  J Mol Appl Genet       Date:  1983

5.  Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.

Authors:  D Valvekens; M Van Montagu; M Van Lijsebettens
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

6.  Characterization of different plaque-forming and defective temperate phages in Agrobacterium.

Authors:  G Vervliet; M Holsters; H Teuchy; M Van Montagu; J Schell
Journal:  J Gen Virol       Date:  1975-01       Impact factor: 3.891

7.  Characterization of the genome of Arabidopsis thaliana.

Authors:  R E Pruitt; E M Meyerowitz
Journal:  J Mol Biol       Date:  1986-01-20       Impact factor: 5.469

8.  Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.

Authors:  S N Sheikholeslam; D P Weeks
Journal:  Plant Mol Biol       Date:  1987-07       Impact factor: 4.076

9.  The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts.

Authors:  P H Schreier; E A Seftor; J Schell; H J Bohnert
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598

10.  High level expression of introduced chimaeric genes in regenerated transformed plants.

Authors:  J D Jones; P Dunsmuir; J Bedbrook
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598
View more
  43 in total

1.  Promoter methylation and progressive transgene inactivation in Arabidopsis.

Authors:  N J Kilby; H M Leyser; I J Furner
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

2.  Genetic transformation of Arabidopsis thaliana zygotic embryos and identification of critical parameters influencing transformation efficiency.

Authors:  R S Sangwan; Y Bourgeois; B S Sangwan-Norreel
Journal:  Mol Gen Genet       Date:  1991-12

3.  Reversible inactivation of a transgene in Arabidopsis thaliana.

Authors:  O Mittelsten Scheid; J Paszkowski; I Potrykus
Journal:  Mol Gen Genet       Date:  1991-08

4.  High efficiency transformation ofBrassica napus usingAgrobacterium vectors.

Authors:  M M Moloney; J M Walker; K K Sharma
Journal:  Plant Cell Rep       Date:  1989-04       Impact factor: 4.570

5.  Agrobacterium-mediated genetic transformation of oilseed Brassica campestris: Transformation frequency is strongly influenced by the mode of shoot regeneration.

Authors:  A Mukhopadhyay; N Arumugam; P B Nandakumar; A K Pradhan; V Gupta; D Pental
Journal:  Plant Cell Rep       Date:  1992-09       Impact factor: 4.570

6.  Arabidopsis thaliana: protocol for plant regeneration from protoplasts.

Authors:  H Karesch; R Bilang; I Potrykus
Journal:  Plant Cell Rep       Date:  1991-02       Impact factor: 4.570

7.  Facile transformation of Arabidopsis.

Authors:  L Márton; J Browse
Journal:  Plant Cell Rep       Date:  1991-08       Impact factor: 4.570

8.  Transformation of Solanum integrifolium poir via Agrobacterium tumefaciens: Plant regeneration and progeny analysis.

Authors:  G L Rotino; D Perrone; P Ajmone-Marsan; E Lupotto
Journal:  Plant Cell Rep       Date:  1992-02       Impact factor: 4.570

9.  Optimizing the production of transformed pea (Pisum sativum L.) callus using disarmed Agrobacterium tumefaciens strains.

Authors:  M M Lulsdorf; H Rempel; J A Jackson; D S Baliski; S L Hobbs
Journal:  Plant Cell Rep       Date:  1991-01       Impact factor: 4.570

10.  Arabidopsis thaliana expressing the cauliflower mosaic virus ORF VI transgene has a late flowering phenotype.

Authors:  C Zijlstra; N Schärer-Hernández; S Gal; T Hohn
Journal:  Virus Genes       Date:  1996       Impact factor: 2.332
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.