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Literature DB >> 24220715

Arabidopsis thaliana: protocol for plant regeneration from protoplasts.

Abstract

We report a protocol for plant regeneration from leaf protoplasts of Arabidopsis thaliana ecotype Zürich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in plant regeneration from protoplast-derived clones is highly variable. In the hands of one of us (H.K.) average shoot regeneration frequency (% of calli regenerating at least one shoot) was ca. 60% and average plant regeneration frequency (% of calli yielding fertile plants in soil) was ca. 40%.

Entities: Species

Year: 1991 PMID： 24220715 DOI： 10.1007/BF00232335

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

9 in total

Review 1. Arabidopsis, a useful weed.

Authors: E M Meyerowitz
Journal: Cell Date: 1989-01-27 Impact factor: 41.582

2. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.

Authors: D Valvekens; M Van Montagu; M Van Lijsebettens
Journal: Proc Natl Acad Sci U S A Date: 1988-08 Impact factor: 11.205

3. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

Authors: A M Lloyd; A R Barnason; S G Rogers; M C Byrne; R T Fraley; R B Horsch
Journal: Science Date: 1986-10-24 Impact factor: 47.728

4. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

5. "Arabidobrassica": A novel plant obtained by protoplast fusion.

Authors: Y Y Gleba; F Hoffmann
Journal: Planta Date: 1980-07 Impact factor: 4.116

6. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

Authors: R Schmidt; L Willmitzer
Journal: Plant Cell Rep Date: 1988-12 Impact factor: 4.570

7. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

Authors: L Menczel; F Nagy; Z R Kiss; P Maliga
Journal: Theor Appl Genet Date: 1981-03 Impact factor: 5.699

8. Gene targeting in plants.

Authors: J Paszkowski; M Baur; A Bogucki; I Potrykus
Journal: EMBO J Date: 1988-12-20 Impact factor: 11.598

9. Direct gene transfer to plants.

Authors: J Paszkowski; R D Shillito; M Saul; V Mandák; T Hohn; B Hohn; I Potrykus
Journal: EMBO J Date: 1984-12-01 Impact factor: 11.598

9 in total

12 in total

7. Plant regeneration from mesophyll-protoplasts of four different ecotypes and two marker lines from Arabidopsis thaliana using a unique protocol.

Authors: J Siemens; M Torres; M Morgner; M D Sacristán
Journal: Plant Cell Rep Date: 1993-08 Impact factor: 4.570

Arabidopsis thaliana: protocol for plant regeneration from protoplasts.

Review 1. Arabidopsis, a useful weed.

2. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.

3. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

4. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

5. "Arabidobrassica": A novel plant obtained by protoplast fusion.

6. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

7. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

8. Gene targeting in plants.

9. Direct gene transfer to plants.

1. Release of epigenetic gene silencing by trans-acting mutations in Arabidopsis.

2. An improved approach for transformation of plant cells by microinjection: molecular and genetic analysis.

3. Reversible inactivation of a transgene in Arabidopsis thaliana.

4. A novel method for regenerating plants from mesophyll protoplasts of Arabidopsis thaliana line WS.

5. Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.).

6. Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana.

7. Plant regeneration from mesophyll-protoplasts of four different ecotypes and two marker lines from Arabidopsis thaliana using a unique protocol.

8. Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens.

9. A simple method for isolation, liquid culture, transformation and regeneration of Arabidopsis thaliana protoplasts.

10. Gene inactivation in Arabidopsis thaliana is not accompanied by an accumulation of repeat-induced point mutations.