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A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Abstract

A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.

Entities: Chemical Disease

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Year: 1978 PMID： 31918 DOI： 10.1016/0005-2744(78)90363-7

Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002

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Cited

140 in total

1. Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.

Authors: D R Goring; L Thomson; S J Rothstein
Journal: Proc Natl Acad Sci U S A Date: 1991-03-01 Impact factor: 11.205

2. Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene.

Authors: J. I. Medford; R. Horgan; Z. El-Sawi; H. J. Klee
Journal: Plant Cell Date: 1989-04 Impact factor: 11.277

3. Constitutive expression of the beta-phaseolin gene in different tissues of transgenic alfalfa does not ensure phaseolin accumulation in non-seed tissue.

Authors: S Bagga; D Sutton; J D Kemp; C Sengupta-Gopalan
Journal: Plant Mol Biol Date: 1992-09 Impact factor: 4.076

4. Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.

Authors: Hetian Lei; Jianjun Qi; Jingyuan Song; Dejun Yang; Yanzhi Wang; Yinling Zhang; Junshan Yang
Journal: Plant Cell Rep Date: 2006-06-07 Impact factor: 4.570

Review 5. Agrobacterium and plant genetic engineering.

Authors: P J Hooykaas; R A Schilperoort
Journal: Plant Mol Biol Date: 1992-05 Impact factor: 4.076

6. Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.

Authors: R B Horsch; H J Klee
Journal: Proc Natl Acad Sci U S A Date: 1986-06 Impact factor: 11.205

A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

1. Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.

2. Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene.

3. Constitutive expression of the beta-phaseolin gene in different tissues of transgenic alfalfa does not ensure phaseolin accumulation in non-seed tissue.

4. Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.

Review 5. Agrobacterium and plant genetic engineering.

6. Rapid assay of foreign gene expression in leaf discs transformed by Agrobacterium tumefaciens: Role of T-DNA borders in the transfer process.

7. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA.

8. The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

9. Agrobacterium vitis nopaline Ti plasmid pTiAB4: relationship to other Ti plasmids and T-DNA structure.

10. Interspecific Gene Transfer (Implications for Broadening Temperature Characteristics of Plant Metabolic Processes).