Literature DB >> 24221548

Agrobacterium-mediated transformation of pepino and regeneration of transgenic plants.

R G Atkinson1, R C Gardner.   

Abstract

Regeneration of pepino (Solanum muricatum Ait.) shoots was achieved both by organogenesis and by embryogenesis. Shoots derived via organogenesis were easily rooted and most regenerated plants appeared phenotypically normal. Transgenic plants were obtained using the binary vector pKIWI110 in the avirulent Agrobacterium tumefaciens strain LBA4404. Optimization of transformation protocols was rapidly achieved by monitoring early expression of the GUS (β-D-glucuronidase) reporter gene carried on pKIWI110. Transgenic plants expressed GUS and selectable marker genes for kanamycin resistance and chlorsulfuron resistance. PCR (polymerase chain reaction) and Southern analysis provided molecular evidence for transformation.

Entities:  

Year:  1991        PMID： 24221548     DOI： 10.1007/BF00234297

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  10 in total

1.  Genetically engineering plants for crop improvement.

Authors:  C S Gasser; R T Fraley
Journal:  Science       Date:  1989-06-16       Impact factor: 47.728

2.  High efficiency transformation ofBrassica napus usingAgrobacterium vectors.

Authors:  M M Moloney; J M Walker; K K Sharma
Journal:  Plant Cell Rep       Date:  1989-04       Impact factor: 4.570

3.  Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium.

Authors:  B J Janssen; R C Gardner
Journal:  Plant Mol Biol       Date:  1990-01       Impact factor: 4.076

4.  A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis.

Authors:  G J Wadsworth; M G Redinbaugh; J G Scandalios
Journal:  Anal Biochem       Date:  1988-07       Impact factor: 3.365

5.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

6.  Transformation of Soybean Cells Using Mixed Strains of Agrobacterium tumefaciens and Phenolic Compounds.

Authors:  L D Owens; A C Smigocki
Journal:  Plant Physiol       Date:  1988-11       Impact factor: 8.340

7.  Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation.

Authors:  G Vancanneyt; R Schmidt; A O'Connor-Sanchez; L Willmitzer; M Rocha-Sosa
Journal:  Mol Gen Genet       Date:  1990-01

8.  Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens.

Authors:  S N Sheikholeslam; D P Weeks
Journal:  Plant Mol Biol       Date:  1987-07       Impact factor: 4.076

9.  High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

Authors:  R Schmidt; L Willmitzer
Journal:  Plant Cell Rep       Date:  1988-12       Impact factor: 4.570

10.  Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation.

Authors:  S C Deroles; R C Gardner
Journal:  Plant Mol Biol       Date:  1988-05       Impact factor: 4.076
  10 in total
  8 in total

1.  The use of transient GUS expression to develop an Agrobacterium-mediated gene transfer system for kiwifruit.

Authors:  B J Janssen; R C Gardner
Journal:  Plant Cell Rep       Date:  1993-11       Impact factor: 4.570

2.  Characterization of transgenic plants derived from hairy roots ofHyoscyamus muticus.

Authors:  N Sevón; B Dräger; R Hiltunen; K -M Oksman-Caldentey
Journal:  Plant Cell Rep       Date:  1997-06       Impact factor: 4.570

3.  Fruit developmental regulation of the kiwifruit actinidin promoter is conserved in transgenic petunia plants.

Authors:  E Lin; D J Burns; R C Gardner
Journal:  Plant Mol Biol       Date:  1993-11       Impact factor: 4.076

4.  Agrobacterium tumefaciens mediated transfer of Phaseolus vulgaris alpha-amylase inhibitor-1 gene into mungbean Vigna radiata (L.) Wilczek using bar as selectable marker.

Authors:  Raman Saini; Rana P Singh; Pawan K Jaiwal
Journal:  Plant Cell Rep       Date:  2006-09-16       Impact factor: 4.570

5.  Highly efficient Agrobacterium-mediated transformation and plant regeneration system for genome engineering in tomato.

Authors:  Dulam Sandhya; Phanikanth Jogam; Ajay Kumar Venkatapuram; Pandarinath Savitikadi; Venkataiah Peddaboina; Venkateswar Rao Allini; Sadanandam Abbagani
Journal:  Saudi J Biol Sci       Date:  2022-04-21       Impact factor: 4.052

6.  Regeneration of transgenic tamarillo plants.

Authors:  R G Atkinson; R C Gardner
Journal:  Plant Cell Rep       Date:  1993-04       Impact factor: 4.570

7.  Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper).

Authors:  Manish Sainger; Darshna Chaudhary; Savita Dahiya; Ranjana Jaiwal; Pawan K Jaiwal
Journal:  Physiol Mol Biol Plants       Date:  2015-07-26

8.  Regeneration of transgenic plants from the commercial apple cultivar Royal Gala.

Authors:  J L Yao; D Cohen; R Atkinson; K Richardson; B Morris
Journal:  Plant Cell Rep       Date:  1995-04       Impact factor: 4.570
  8 in total

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