Literature DB >> 2461112

A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis.

G J Wadsworth1, M G Redinbaugh, J G Scandalios.   

Abstract

A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.

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Year:  1988        PMID： 2461112     DOI： 10.1016/0003-2697(88)90443-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  82 in total

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