Literature DB >> 24221382

Molecular characterization of a low-molecular-weight glutenin cDNA clone from Triticum durum.

B G Cassidy1, J Dvorak.   

Abstract

A full-length, low-molecular-weight (LMW) glutenin cDNA clone, pTdUCD1, has been isolated from a Triticum durum cv 'Mexicali' wheat cDNA library. The complete sequence was determined and compared to the LMW glutenin genes that have been isolated from hexaploid wheat, Triticum aestivum. This cDNA codes for a protein of 295 amino acids (33,414 daltons) including a 20-amino acid signal peptide as deduced from the DNA sequence. Northern analysis showed that this cDNA hybridizes to a family of related sequences ranging in length from 1,200 to 1,000 nucleotides. This gene is similar but not identical to previously published LMW glutenin gene sequences. The most striking characteristic of all cloned LMW glutenin genes is the conservation of eight cysteine residues, which could be involved in potential secondary or tertiary structure, disulfide bond interactions. This paper presents a structural map defining distinct regions of the LMW glutenin gene family.

Entities:  

Year:  1991        PMID： 24221382     DOI： 10.1007/BF00226733

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  13 in total

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7.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

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9.  DNA sequencing with chain-terminating inhibitors.

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Authors:  P I Payne; K G Corfield; J A Blackman
Journal:  Theor Appl Genet       Date:  1979-05       Impact factor: 5.699
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  9 in total

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5.  Construction and characterization of a half million clone BAC library of durum wheat ( Triticum turgidum ssp. durum).

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9.  Rapid genome mapping in nanochannel arrays for highly complete and accurate de novo sequence assembly of the complex Aegilops tauschii genome.

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  9 in total

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