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Literature DB >> 6190127

The characterization of cDNA clones coding for wheat storage proteins.

Abstract

Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley storage protein, B-hordein. The sequence includes a tandem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.

Entities: Chemical Species

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Year: 1983 PMID： 6190127 PMCID： PMC325941 DOI： 10.1093/nar/11.10.2961

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

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2. Plasmid detection and sizing in single colony lysates.

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3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

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Authors: W M Bonner; R A Laskey
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5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors: P S Thomas
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

6. The ovalbumin gene-sequence of putative control regions.

Authors: C Benoist; K O'Hare; R Breathnach; P Chambon
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7. Cloning and sequence analysis reveal structural variation among related zein genes in maize.

Authors: K Pedersen; J Devereux; D R Wilson; E Sheldon; B A Larkins
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8. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

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9. A membrane-filter technique for the detection of complementary DNA.

Authors: D T Denhardt
Journal: Biochem Biophys Res Commun Date: 1966-06-13 Impact factor: 3.575

10. Cloning and characterization of ribosomal RNA genes from wheat and barley.

Authors: W L Gerlach; J R Bedbrook
Journal: Nucleic Acids Res Date: 1979-12-11 Impact factor: 16.971

55 in total

1. Floral genes expressed in tomato hypocotyl explants in liquid culture.

Authors: L Pitto; L Giorgetti; A Turrini; M Evangelista; G Luccarini; C Colella; F Collina; L Caltavuturo; V Nuti Ronchi
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2. Characterization of SP1, a stress-responsive, boiling-soluble, homo-oligomeric protein from aspen.

Authors: Wang-Xia Wang; Dan Pelah; Tal Alergand; Oded Shoseyov; Arie Altman
Journal: Plant Physiol Date: 2002-10 Impact factor: 8.340

3. PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).

Authors: Xiaofei Zhang; Dongcheng Liu; Wei Jiang; Xiaoli Guo; Wenlong Yang; Jiazhu Sun; Hongqing Ling; Aimin Zhang
Journal: Theor Appl Genet Date: 2011-08-10 Impact factor: 5.699

The characterization of cDNA clones coding for wheat storage proteins.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. Plasmid detection and sizing in single colony lysates.

3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

6. The ovalbumin gene-sequence of putative control regions.

7. Cloning and sequence analysis reveal structural variation among related zein genes in maize.

8. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

9. A membrane-filter technique for the detection of complementary DNA.

10. Cloning and characterization of ribosomal RNA genes from wheat and barley.

1. Floral genes expressed in tomato hypocotyl explants in liquid culture.

2. Characterization of SP1, a stress-responsive, boiling-soluble, homo-oligomeric protein from aspen.

3. PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).

4. Sequence variability and gene structure at the self-incompatibility locus of Solanum tuberosum.

5. Nucleotide sequence of a low-molecular-weight glutenin from Triticum durum.

Review 6. Molecular biology and coeliac disease.

7. Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells.

Review 8. Cereal chemistry, molecular biology, and toxicity in coeliac disease.

9. cDNA cloning, characterization and expression of an endosperm-specific barley peroxidase.

10. GA3-regulated cDNAs from Hordeum vulgare leaves.