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Literature DB >> 24221308

Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

P A Lazzeri¹, R Brettschneider, R Lührs, H Lörz.

Abstract

Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and β-glucuronidase (GUS) genes, using polyethyleneglycol (PEG) to induce DNA uptake. Transformed microcalli were selected in media containing G418 sulphate. NPT activity was detected in all antibiotic-resistant cell lines, but not all NPT-positive cell lines had GUS activity. Southern analysis confirmed the presence of sequences homologous to the APT and GUS genes in DNA of G418-resistant callus.

Entities: Chemical Species

Year: 1991 PMID： 24221308 DOI： 10.1007/BF00219433

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

20 in total

1. Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake.

Authors: B Junker; J Zimny; R Lührs; H Lörz
Journal: Plant Cell Rep Date: 1987-10 Impact factor: 4.570

2. Transfection of germinating barley seed electrophoretically with exogenous DNA.

Authors: H Ahokas
Journal: Theor Appl Genet Date: 1989-04 Impact factor: 5.699

3. Co-transformation of indica rice protoplasts with gusA and neo genes.

Authors: J Peng; L A Lyznik; L Lee; T K Hodges
Journal: Plant Cell Rep Date: 1990-07 Impact factor: 4.570

4. Genetically transformed maize plants from protoplasts.

Authors: C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal: Science Date: 1988-04-08 Impact factor: 47.728

5. Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat.

Authors: B Lee; K Murdoch; J Topping; M Kreis; M G Jones
Journal: Plant Mol Biol Date: 1989-07 Impact factor: 4.076

6. Agrobacterium - and microprojectile - mediated viral DNA delivery into barley microspore-derived cultures.

Authors: G Creissen; C Smith; R Francis; H Reynolds; P Mullineaux
Journal: Plant Cell Rep Date: 1990-04 Impact factor: 4.570

7. Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment.

Authors: K K Kartha; R N Chibbar; F Georges; N Leung; K Caswell; E Kendall; J Qureshi
Journal: Plant Cell Rep Date: 1989-12 Impact factor: 4.570

8. Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Authors: W. J. Gordon-Kamm; T. M. Spencer; M. L. Mangano; T. R. Adams; R. J. Daines; W. G. Start; J. V. O'Brien; S. A. Chambers; W. R. Adams; N. G. Willetts; T. B. Rice; C. J. Mackey; R. W. Krueger; A. P. Kausch; P. G. Lemaux
Journal: Plant Cell Date: 1990-07 Impact factor: 11.277

9. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

10. Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

Authors: H Yang; H M Zhang; M R Davey; B J Mulligan; E C Cocking
Journal: Plant Cell Rep Date: 1988-10 Impact factor: 4.570

28 in total

1. Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination.

Authors: A M Nuutila; A Ritala; R W Skadsen; L Mannonen; V Kauppinen
Journal: Plant Mol Biol Date: 1999-12 Impact factor: 4.076

Review 2. Transgene silencing in monocots.

Authors: L M Iyer; S P Kumpatla; M B Chandrasekharan; T C Hall
Journal: Plant Mol Biol Date: 2000-06 Impact factor: 4.076

Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

1. Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake.

2. Transfection of germinating barley seed electrophoretically with exogenous DNA.

3. Co-transformation of indica rice protoplasts with gusA and neo genes.

4. Genetically transformed maize plants from protoplasts.

5. Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat.

6. Agrobacterium - and microprojectile - mediated viral DNA delivery into barley microspore-derived cultures.

7. Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment.

8. Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

9. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

10. Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

1. Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination.

Review 2. Transgene silencing in monocots.

3. Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags.

4. Plant regeneration from protoplasts of wild barley (Hordeum murinum L.).

5. Regeneration of transgenic, microspore-derived, fertile barley.

6. Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.).

7. Primary callus as source of totipotent barley (Hordeum vulgare L.) protoplasts.

8. Isolation of protoplasts from developing barley endosperm: a tool for transient expression studies.

9. Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.).

10. Chromosomal variation in dividing protoplasts derived from cell suspensions of barley (Hordeum vulgare L.).