Literature DB >> 24232711

Transfection of germinating barley seed electrophoretically with exogenous DNA.

H Ahokas1.   

Abstract

A method is described for transfection (genetic transformation) of barley caryopsis electrophoretically with DNA. β-Glucuronidase activity was detected after the electrophoretic transfection with plasmid pBI221 DNA carrying the cauliflower mosaic virus promotor and bacterial β-glucuronidase coding sequence. Electrophoretic transfection is evidently effective with pieces of callus and seeds of many plants.

Entities:  

Year:  1989        PMID： 24232711     DOI： 10.1007/BF00274265

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  14 in total

1.  Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum.

Authors:  T M Ou-Lee; R Turgeon; R Wu
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

2.  Stable transformation of maize after gene transfer by electroporation.

Authors:  M E Fromm; L P Taylor; V Walbot
Journal:  Nature       Date:  1986 Feb 27-Mar 5       Impact factor: 49.962

3.  A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.

Authors:  L A Otten; R A Schilperoort
Journal:  Biochim Biophys Acta       Date:  1978-12-08

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Opine synthesis in wild-type plant tissue.

Authors:  P Christou; S G Platt; M C Ackerman
Journal:  Plant Physiol       Date:  1986-09       Impact factor: 8.340

6.  Gibberellic Acid enhancement of DNA turnover in barley aleurone cells.

Authors:  L Taiz; J E Starks
Journal:  Plant Physiol       Date:  1977-08       Impact factor: 8.340

7.  Barley aleurone layers secrete a nuclease in response to gibberellic Acid : purification and partial characterization of the associated ribonuclease, deoxyribonuclease, and 3'-nucleotidase activities.

Authors:  P H Brown; T H Ho
Journal:  Plant Physiol       Date:  1986-11       Impact factor: 8.340

8.  beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors:  R A Jefferson; S M Burgess; D Hirsh
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

9.  Liposome-mediated delivery of DNA to carrot protoplasts.

Authors:  B F Matthews; D E Cress
Journal:  Planta       Date:  1981-10       Impact factor: 4.116

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  3 in total

1.  Electroporation-mediated DNA delivery to seedling tissues ofPhaseolus vulgaris L. (common bean).

Authors:  W Dillen; G Engler; M Van Montagu; G Angenon
Journal:  Plant Cell Rep       Date:  1995-01       Impact factor: 4.570

Review 2.  Gene transfer by electroporation.

Authors:  P F Lurquin
Journal:  Mol Biotechnol       Date:  1997-02       Impact factor: 2.695

3.  Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

Authors:  P A Lazzeri; R Brettschneider; R Lührs; H Lörz
Journal:  Theor Appl Genet       Date:  1991-04       Impact factor: 5.699
  3 in total

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