Literature DB >> 24177904

Regeneration of transgenic, microspore-derived, fertile barley.

A Jähne1, D Becker, R Brettschneider, H Lörz.   

Abstract

We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue. Independent transformation events led, on average, to the recovery of one plant per 1×10(7) bombarded microspores. Putative transformants have been regenerated using phosphinothricin as a selective agent. R0 plants have been transferred to soil approximately 2 months after bombardment. Integration of the marker genes bar and uidA has been confirmed by Southern analysis. The marker genes are inherited in all progeny plants confirming the expected homozygous nature of the R0 plants.

Entities:  

Year:  1994        PMID： 24177904     DOI： 10.1007/BF00225390

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  18 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Microspore culture of Hordeum vulgare L.: the influence of density and osmolality.

Authors:  S Hoekstra; M H van Zijderveld; F Heidekamp; F van der Mark
Journal:  Plant Cell Rep       Date:  1993-10       Impact factor: 4.570

3.  Electroporation and PEG delivery of DNA into maize microspores.

Authors:  A Fennell; R Hauptmann
Journal:  Plant Cell Rep       Date:  1992-10       Impact factor: 4.570

4.  Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.).

Authors:  A Jähne; P A Lazzeri; M Jäger-Gussen; H Lörz
Journal:  Theor Appl Genet       Date:  1991-07       Impact factor: 5.699

5.  Production of doubled haploid lines in frequencies sufficient for barley breeding programs.

Authors:  U Kuhlmann; B Foroughi-Wehr
Journal:  Plant Cell Rep       Date:  1989-02       Impact factor: 4.570

6.  Protoplast preparation without centrifugation: plant regeneration of barley (Hordeum vulgare L.).

Authors:  T J Golds; J Babczinsky; A P Mordhorst; H U Koop
Journal:  Plant Cell Rep       Date:  1994-01       Impact factor: 4.570

7.  Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Authors:  W. J. Gordon-Kamm; T. M. Spencer; M. L. Mangano; T. R. Adams; R. J. Daines; W. G. Start; J. V. O'Brien; S. A. Chambers; W. R. Adams; N. G. Willetts; T. B. Rice; C. J. Mackey; R. W. Krueger; A. P. Kausch; P. G. Lemaux
Journal:  Plant Cell       Date:  1990-07       Impact factor: 11.277

8.  Fertile transgenic wheat from microprojectile bombardment of scutellar tissue.

Authors:  D Becker; R Brettschneider; H Lörz
Journal:  Plant J       Date:  1994-02       Impact factor: 6.417

9.  Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

Authors:  P A Lazzeri; R Brettschneider; R Lührs; H Lörz
Journal:  Theor Appl Genet       Date:  1991-04       Impact factor: 5.699

10.  Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgare L.).

Authors:  F L Olsen
Journal:  Hereditas       Date:  1991       Impact factor: 3.271
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  15 in total

1.  Expression of fungal thermotolerant endo-1,4-beta-glucanase in transgenic barley seeds during germination.

Authors:  A M Nuutila; A Ritala; R W Skadsen; L Mannonen; V Kauppinen
Journal:  Plant Mol Biol       Date:  1999-12       Impact factor: 4.076

2.  Gametic embryos of maize as a target for biolistic transformation: comparison to immature zygotic embryos.

Authors:  I E Aulinger; S O Peter; J E Schmid; P Stamp
Journal:  Plant Cell Rep       Date:  2002-12-17       Impact factor: 4.570

3.  Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation.

Authors:  Mette Lange; Eva Vincze; Marianne G Møller; Preben B Holm
Journal:  Plant Cell Rep       Date:  2006-03-10       Impact factor: 4.570

4.  Genetic Transformation of Hordeum vulgare ssp. spontaneum for the Development of a Transposon-Based Insertional Mutagenesis System.

Authors:  Marie-Josée Cardinal; Rajvinder Kaur; Jaswinder Singh
Journal:  Mol Biotechnol       Date:  2016-10       Impact factor: 2.695

5.  Albino Plant Formation in Androgenic Cultures: An Old Problem and New Facts.

Authors:  Iwona Żur; Monika Gajecka; Ewa Dubas; Monika Krzewska; Iwona Szarejko
Journal:  Methods Mol Biol       Date:  2021

6.  Methotrexate is a new selectable marker for tobacco immature pollen transformation.

Authors:  Tatiana Aionesei; Julia Hosp; Viktor Voronin; Erwin Heberle-Bors; Alisher Touraev
Journal:  Plant Cell Rep       Date:  2005-12-06       Impact factor: 4.570

7.  The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley ( Hordeum vulgare L) uninucleate microspores.

Authors:  Y S Shim; K J Kasha
Journal:  Plant Cell Rep       Date:  2003-04-29       Impact factor: 4.570

8.  Long-term stability of transgene expression driven by barley endosperm-specific hordein promoters in transgenic barley.

Authors:  H W Choi; P G Lemaux; M-J Cho
Journal:  Plant Cell Rep       Date:  2003-04-29       Impact factor: 4.570

9.  Mapping Ds insertions in barley using a sequence-based approach.

Authors:  L D Cooper; L Marquez-Cedillo; J Singh; A K Sturbaum; S Zhang; V Edwards; K Johnson; A Kleinhofs; S Rangel; V Carollo; P Bregitzer; P G Lemaux; P M Hayes
Journal:  Mol Genet Genomics       Date:  2004-07-30       Impact factor: 3.291

10.  An Ac/Ds-mediated gene trap system for functional genomics in barley.

Authors:  Katina Lazarow; Stephanie Lütticke
Journal:  BMC Genomics       Date:  2009-01-29       Impact factor: 3.969
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