Literature DB >> 24196247

Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement.

T Altmann1, B Damm, W B Frommer, T Martin, P C Morris, D Schweizer, L Willmitzer, R Schmidt.   

Abstract

Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

Entities:  

Year:  1994        PMID： 24196247     DOI： 10.1007/BF00232939

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  15 in total

1.  High-frequency T-DNA-mediated gene tagging in plants.

Authors:  C Koncz; N Martini; R Mayerhofer; Z Koncz-Kalman; H Körber; G P Redei; J Schell
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

2.  Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.

Authors:  W. Jahnen; W. M. Lush; A. E. Clarke
Journal:  Plant Cell       Date:  1989-05       Impact factor: 11.277

Review 3.  Arabidopsis, a useful weed.

Authors:  E M Meyerowitz
Journal:  Cell       Date:  1989-01-27       Impact factor: 41.582

4.  Genetic transformation of Arabidopsis thaliana zygotic embryos and identification of critical parameters influencing transformation efficiency.

Authors:  R S Sangwan; Y Bourgeois; B S Sangwan-Norreel
Journal:  Mol Gen Genet       Date:  1991-12

5.  Ploidy levels in transgenic tomato plants determined by chloroplast number.

Authors:  J P Jacobs; J I Yoder
Journal:  Plant Cell Rep       Date:  1989-03       Impact factor: 4.570

6.  Direct gene transfer to protoplasts of Arabidopsis thaliana.

Authors:  H Karesch; R Bilang; O Mittelsten Scheid; I Potrykus
Journal:  Plant Cell Rep       Date:  1991-02       Impact factor: 4.570

7.  Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors:  B Damm; R Schmidt; L Willmitzer
Journal:  Mol Gen Genet       Date:  1989-05

8.  Phytochrome control of the tms2 gene in transgenic Arabidopsis: a strategy for selecting mutants in the signal transduction pathway.

Authors:  G A Karlin-Neumann; J A Brusslan; E M Tobin
Journal:  Plant Cell       Date:  1991-06       Impact factor: 11.277

9.  Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

Authors:  A M Lloyd; A R Barnason; S G Rogers; M C Byrne; R T Fraley; R B Horsch
Journal:  Science       Date:  1986-10-24       Impact factor: 47.728

10.  High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants.

Authors:  R Schmidt; L Willmitzer
Journal:  Plant Cell Rep       Date:  1988-12       Impact factor: 4.570
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  15 in total

1.  Aberrant Classopollis pollen reveals evidence for unreduced (2n) pollen in the conifer family Cheirolepidiaceae during the Triassic-Jurassic transition.

Authors:  Wolfram M Kürschner; Sietske J Batenburg; Luke Mander
Journal:  Proc Biol Sci       Date:  2013-08-07       Impact factor: 5.349

2.  Volume-based pollen size analysis: an advanced method to assess somatic and gametophytic ploidy in flowering plants.

Authors:  Nico De Storme; Linda Zamariola; Martin Mau; Timothy F Sharbel; Danny Geelen
Journal:  Plant Reprod       Date:  2013-01-11       Impact factor: 3.767

3.  Evaluation of DNA damage in tobacco male meiocytes involved in cytomixis using comet assay.

Authors:  Sergey Mursalimov; Alla Zagorskaya; Elena Deineko
Journal:  Protoplasma       Date:  2017-07-12       Impact factor: 3.356

4.  The Arabidopsis mutant jason produces unreduced first division restitution male gametes through a parallel/fused spindle mechanism in meiosis II.

Authors:  Nico De Storme; Danny Geelen
Journal:  Plant Physiol       Date:  2011-01-21       Impact factor: 8.340

5.  Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana.

Authors:  S Holtorf; K Apel; H Bohlmann
Journal:  Plant Mol Biol       Date:  1995-11       Impact factor: 4.076

Review 6.  Cytogenetic tools for Arabidopsis thaliana.

Authors:  Maarten Koornneef; Paul Fransz; Hans de Jong
Journal:  Chromosome Res       Date:  2003       Impact factor: 5.239

7.  GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 are required for ploidy consistency of the sexual reproduction system in Arabidopsis.

Authors:  Nico De Storme; Joachim De Schrijver; Wim Van Criekinge; Vera Wewer; Peter Dörmann; Danny Geelen
Journal:  Plant Cell       Date:  2013-02-12       Impact factor: 11.277

8.  A large number of tetraploid Arabidopsis thaliana lines, generated by a rapid strategy, reveal high stability of neo-tetraploids during consecutive generations.

Authors:  Zheng Yu; Kristina Haage; Verena E Streit; Alfons Gierl; Ramón A Torres Ruiz
Journal:  Theor Appl Genet       Date:  2009-02-11       Impact factor: 5.699

9.  Cytological events in explants of Arabidopsis thaliana during early callogenesis.

Authors:  A Fras; J Juchimiuk; D Siwinska; J Maluszynska
Journal:  Plant Cell Rep       Date:  2007-07-26       Impact factor: 4.570

10.  Imprinting of the polycomb group gene MEDEA serves as a ploidy sensor in Arabidopsis.

Authors:  Aleksandra Erilova; Lynette Brownfield; Vivien Exner; Marisa Rosa; David Twell; Ortrun Mittelsten Scheid; Lars Hennig; Claudia Köhler
Journal:  PLoS Genet       Date:  2009-09-25       Impact factor: 5.917
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