Literature DB >> 24173663

Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests.

Liangliang Sun1, Guijie Zhu, Yimeng Zhao, Xiaojing Yan, Si Mou, Norman J Dovichi.   

Abstract

Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  electrophoresis; electrospray; mass spectrometry; proteomics; ultrasensitive analysis

Mesh:

Substances:

Year:  2013        PMID: 24173663      PMCID: PMC3904452          DOI: 10.1002/anie.201308139

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN: 1433-7851            Impact factor:   15.336


  23 in total

1.  An accurate mass tag strategy for quantitative and high-throughput proteome measurements.

Authors:  Richard D Smith; Gordon A Anderson; Mary S Lipton; Ljiljana Pasa-Tolic; Yufeng Shen; Thomas P Conrads; Timothy D Veenstra; Harold R Udseth
Journal:  Proteomics       Date:  2002-05       Impact factor: 3.984

2.  Ultrasensitive proteomics using high-efficiency on-line micro-SPE-nanoLC-nanoESI MS and MS/MS.

Authors:  Yufeng Shen; Nikola Tolić; Christophe Masselon; Ljiljana Pasa-Tolić; David G Camp; Kim K Hixson; Rui Zhao; Gordon A Anderson; Richard D Smith
Journal:  Anal Chem       Date:  2004-01-01       Impact factor: 6.986

3.  Simplifying CE-MS operation. 2. Interfacing low-flow separation techniques to mass spectrometry using a porous tip.

Authors:  Mehdi Moini
Journal:  Anal Chem       Date:  2007-04-21       Impact factor: 6.986

Review 4.  Applying mass spectrometry-based proteomics to genetics, genomics and network biology.

Authors:  Matthias Gstaiger; Ruedi Aebersold
Journal:  Nat Rev Genet       Date:  2009-09       Impact factor: 53.242

5.  Comparison of the LTQ-Orbitrap Velos and the Q-Exactive for proteomic analysis of 1-1000 ng RAW 264.7 cell lysate digests.

Authors:  Liangliang Sun; Guijie Zhu; Norman J Dovichi
Journal:  Rapid Commun Mass Spectrom       Date:  2013-01-15       Impact factor: 2.419

Review 6.  Protein analysis by shotgun/bottom-up proteomics.

Authors:  Yaoyang Zhang; Bryan R Fonslow; Bing Shan; Moon-Chang Baek; John R Yates
Journal:  Chem Rev       Date:  2013-02-26       Impact factor: 60.622

7.  Single-shot proteomics using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry with production of more than 1250 Escherichia coli peptide identifications in a 50 min separation.

Authors:  Guijie Zhu; Liangliang Sun; Xiaojing Yan; Norman J Dovichi
Journal:  Anal Chem       Date:  2013-02-13       Impact factor: 6.986

Review 8.  High sensitivity capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for the rapid analysis of complex proteomes.

Authors:  Liangliang Sun; Guijie Zhu; Xiaojing Yan; Norman J Dovichi
Journal:  Curr Opin Chem Biol       Date:  2013-08-01       Impact factor: 8.822

9.  On-line 1D and 2D porous layer open tubular/LC-ESI-MS using 10-microm-i.d. poly(styrene-divinylbenzene) columns for ultrasensitive proteomic analysis.

Authors:  Quanzhou Luo; Guihua Yue; Gary A Valaskovic; Ye Gu; Shiaw-Lin Wu; Barry L Karger
Journal:  Anal Chem       Date:  2007-07-11       Impact factor: 6.986

10.  PaxDb, a database of protein abundance averages across all three domains of life.

Authors:  M Wang; M Weiss; M Simonovic; G Haertinger; S P Schrimpf; M O Hengartner; C von Mering
Journal:  Mol Cell Proteomics       Date:  2012-04-24       Impact factor: 5.911

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  79 in total

Review 1.  New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells.

Authors:  Sneha P Couvillion; Ying Zhu; Gabe Nagy; Joshua N Adkins; Charles Ansong; Ryan S Renslow; Paul D Piehowski; Yehia M Ibrahim; Ryan T Kelly; Thomas O Metz
Journal:  Analyst       Date:  2019-01-28       Impact factor: 4.616

2.  Sensitive and fast characterization of site-specific protein glycosylation with capillary electrophoresis coupled to mass spectrometry.

Authors:  Yanyan Qu; Liangliang Sun; Guijie Zhu; Zhenbin Zhang; Elizabeth H Peuchen; Norman J Dovichi
Journal:  Talanta       Date:  2017-10-31       Impact factor: 6.057

3.  Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Phosphoproteomics with the Production of over 11,000 Phosphopeptides from the Colon Carcinoma HCT116 Cell Line.

Authors:  Daoyang Chen; Katelyn R Ludwig; Oleg V Krokhin; Vic Spicer; Zhichang Yang; Xiaojing Shen; Amanda B Hummon; Liangliang Sun
Journal:  Anal Chem       Date:  2019-01-23       Impact factor: 6.986

4.  Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome.

Authors:  Elijah N McCool; Rachele A Lubeckyj; Xiaojing Shen; Daoyang Chen; Qiang Kou; Xiaowen Liu; Liangliang Sun
Journal:  Anal Chem       Date:  2018-04-09       Impact factor: 6.986

5.  Capillary electrophoresis coupled to negative mode electrospray ionization-mass spectrometry using an electrokinetically-pumped nanospray interface with primary amines grafted to the interior of a glass emitter.

Authors:  Scott A Sarver; Nicole M Schiavone; Jennifer Arceo; Elizabeth H Peuchen; Zhenbin Zhang; Liangliang Sun; Norman J Dovichi
Journal:  Talanta       Date:  2017-01-03       Impact factor: 6.057

6.  Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.

Authors:  Ying Zhu; Geremy Clair; William B Chrisler; Yufeng Shen; Rui Zhao; Anil K Shukla; Ronald J Moore; Ravi S Misra; Gloria S Pryhuber; Richard D Smith; Charles Ansong; Ryan T Kelly
Journal:  Angew Chem Int Ed Engl       Date:  2018-06-14       Impact factor: 15.336

7.  Rapid and simple isolation of vascular, epidermal and mesophyll cells from plant leaf tissue.

Authors:  Motomu Endo; Hanako Shimizu; Takashi Araki
Journal:  Nat Protoc       Date:  2016-07-07       Impact factor: 13.491

Review 8.  Recent Advances in the Analysis of Complex Glycoproteins.

Authors:  Stefan Gaunitz; Gabe Nagy; Nicola L B Pohl; Milos V Novotny
Journal:  Anal Chem       Date:  2016-11-23       Impact factor: 6.986

9.  High speed capillary zone electrophoresis-mass spectrometry via an electrokinetically pumped sheath flow interface for rapid analysis of amino acids and a protein digest.

Authors:  Nicole M Schiavone; Scott A Sarver; Liangliang Sun; Roza Wojcik; Norman J Dovichi
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2015-04-09       Impact factor: 3.205

Review 10.  Capillary zone electrophoresis as a tool for bottom-up protein analysis.

Authors:  Roza Wojcik; Guijie Zhu; Zhenbin Zhang; Xiaojing Yan; Yimeng Zhao; Liangliang Sun; Matthew M Champion; Norman J Dovichi
Journal:  Bioanalysis       Date:  2015-12-10       Impact factor: 2.681

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