Literature DB >> 29797682

Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.

Ying Zhu1, Geremy Clair2, William B Chrisler2, Yufeng Shen2, Rui Zhao1, Anil K Shukla2, Ronald J Moore2, Ravi S Misra3, Gloria S Pryhuber3, Richard D Smith2, Charles Ansong2, Ryan T Kelly1.   

Abstract

We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was employed to deposit cells into a newly developed nanodroplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC-MS. An average of circa 670 protein groups were confidently identified from single HeLa cells, which is a far greater level of proteome coverage for single cells than has been previously reported. We demonstrate that the single-cell proteomics platform can be used to differentiate cell types from enzyme-dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  cell typing; droplets; microfluidics; single-cell proteomics; ultrasensitive LC-MS

Mesh：

Substances：

Year:  2018        PMID： 29797682      PMCID： PMC6261339          DOI： 10.1002/anie.201802843

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  31 in total

Review 1.  Ultrasensitive and quantitative analyses from combined separations-mass spectrometry for the characterization of proteomes.

Authors:  Richard D Smith; Yufeng Shen; Keqi Tang
Journal:  Acc Chem Res       Date:  2004-04       Impact factor: 22.384

Review 2.  Trends in ultrasensitive proteomics.

Authors:  A F Maarten Altelaar; Albert J R Heck
Journal:  Curr Opin Chem Biol       Date:  2012-01-07       Impact factor: 8.822

3.  An Integrated Platform for Isolation, Processing, and Mass Spectrometry-based Proteomic Profiling of Rare Cells in Whole Blood.

Authors:  Siyang Li; Brian D Plouffe; Arseniy M Belov; Somak Ray; Xianzhe Wang; Shashi K Murthy; Barry L Karger; Alexander R Ivanov
Journal:  Mol Cell Proteomics       Date:  2015-03-09       Impact factor: 5.911

4.  Ultrasensitive Proteome Profiling for 100 Living Cells by Direct Cell Injection, Online Digestion and Nano-LC-MS/MS Analysis.

Authors:  Qi Chen; Guoquan Yan; Mingxia Gao; Xiangmin Zhang
Journal:  Anal Chem       Date:  2015-06-23       Impact factor: 6.986

5.  The coming age of complete, accurate, and ubiquitous proteomes.

Authors:  Matthias Mann; Nils A Kulak; Nagarjuna Nagaraj; Jürgen Cox
Journal:  Mol Cell       Date:  2013-02-21       Impact factor: 17.970

6.  Visualization and analysis of gene expression in tissue sections by spatial transcriptomics.

Authors:  Patrik L Ståhl; Fredrik Salmén; Sanja Vickovic; Anna Lundmark; José Fernández Navarro; Jens Magnusson; Stefania Giacomello; Michaela Asp; Jakub O Westholm; Mikael Huss; Annelie Mollbrink; Sten Linnarsson; Simone Codeluppi; Åke Borg; Fredrik Pontén; Paul Igor Costea; Pelin Sahlén; Jan Mulder; Olaf Bergmann; Joakim Lundeberg; Jonas Frisén
Journal:  Science       Date:  2016-07-01       Impact factor: 47.728

7.  Ultra-High Resolution Ion Mobility Separations Utilizing Traveling Waves in a 13 m Serpentine Path Length Structures for Lossless Ion Manipulations Module.

Authors:  Liulin Deng; Yehia M Ibrahim; Ahmed M Hamid; Sandilya V B Garimella; Ian K Webb; Xueyun Zheng; Spencer A Prost; Jeremy A Sandoval; Randolph V Norheim; Gordon A Anderson; Aleksey V Tolmachev; Erin S Baker; Richard D Smith
Journal:  Anal Chem       Date:  2016-08-26       Impact factor: 6.986

8.  Development of mass spectrometry-based shotgun method for proteome analysis of 500 to 5000 cancer cells.

Authors:  Nan Wang; Mingguo Xu; Peng Wang; Liang Li
Journal:  Anal Chem       Date:  2010-03-15       Impact factor: 6.986

9.  Single-cell spatial reconstruction reveals global division of labour in the mammalian liver.

Authors:  Keren Bahar Halpern; Rom Shenhav; Orit Matcovitch-Natan; Beata Toth; Doron Lemze; Matan Golan; Efi E Massasa; Shaked Baydatch; Shanie Landen; Andreas E Moor; Alexander Brandis; Amir Giladi; Avigail Stokar Avihail; Eyal David; Ido Amit; Shalev Itzkovitz
Journal:  Nature       Date:  2017-02-06       Impact factor: 49.962

10.  Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ.

Authors:  Jürgen Cox; Marco Y Hein; Christian A Luber; Igor Paron; Nagarjuna Nagaraj; Matthias Mann
Journal:  Mol Cell Proteomics       Date:  2014-06-17       Impact factor: 5.911
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  47 in total

Review 1.  New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells.

Authors:  Sneha P Couvillion; Ying Zhu; Gabe Nagy; Joshua N Adkins; Charles Ansong; Ryan S Renslow; Paul D Piehowski; Yehia M Ibrahim; Ryan T Kelly; Thomas O Metz
Journal:  Analyst       Date:  2019-01-28       Impact factor: 4.616

2.  Automated Coupling of Nanodroplet Sample Preparation with Liquid Chromatography-Mass Spectrometry for High-Throughput Single-Cell Proteomics.

Authors:  Sarah M Williams; Andrey V Liyu; Chia-Feng Tsai; Ronald J Moore; Daniel J Orton; William B Chrisler; Matthew J Gaffrey; Tao Liu; Richard D Smith; Ryan T Kelly; Ljiljana Pasa-Tolic; Ying Zhu
Journal:  Anal Chem       Date:  2020-07-22       Impact factor: 6.986

3.  Benchtop-compatible sample processing workflow for proteome profiling of < 100 mammalian cells.

Authors:  Kerui Xu; Yiran Liang; Paul D Piehowski; Maowei Dou; Kaitlynn C Schwarz; Rui Zhao; Ryan L Sontag; Ronald J Moore; Ying Zhu; Ryan T Kelly
Journal:  Anal Bioanal Chem       Date:  2018-11-20       Impact factor: 4.142

Review 4.  Spatiotemporal changes in the human lens proteome: Critical insights into long-lived proteins.

Authors:  Kevin L Schey; Zhen Wang; Michael G Friedrich; Donita L Garland; Roger J W Truscott
Journal:  Prog Retin Eye Res       Date:  2019-11-06       Impact factor: 21.198

Review 5.  Nano-liquid chromatography-mass spectrometry and recent applications in omics investigations.

Authors:  Katherine L Sanders; James L Edwards
Journal:  Anal Methods       Date:  2020-09-09       Impact factor: 2.896

Review 6.  Single-cell Proteomics: Progress and Prospects.

Authors:  Ryan T Kelly
Journal:  Mol Cell Proteomics       Date:  2020-08-26       Impact factor: 5.911

7.  Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells.

Authors:  Kendall Martin; Tong Zhang; Pengfei Zhang; William B Chrisler; Fillmore L Thomas; Fen Liu; Tao Liu; Wei-Jun Qian; Richard D Smith; Tujin Shi
Journal:  J Vis Exp       Date:  2020-11-06       Impact factor: 1.355

8.  Carrier-Assisted Single-Tube Processing Approach for Targeted Proteomics Analysis of Low Numbers of Mammalian Cells.

Authors:  Pengfei Zhang; Matthew J Gaffrey; Ying Zhu; William B Chrisler; Thomas L Fillmore; Lian Yi; Carrie D Nicora; Tong Zhang; Huanming Wu; Jon Jacobs; Keqi Tang; Jacob Kagan; Sudhir Srivastava; Karin D Rodland; Wei-Jun Qian; Richard D Smith; Tao Liu; H Steven Wiley; Tujin Shi
Journal:  Anal Chem       Date:  2018-12-28       Impact factor: 6.986

9.  Improved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry.

Authors:  Yongzheng Cong; Yiran Liang; Khatereh Motamedchaboki; Romain Huguet; Thy Truong; Rui Zhao; Yufeng Shen; Daniel Lopez-Ferrer; Ying Zhu; Ryan T Kelly
Journal:  Anal Chem       Date:  2020-01-21       Impact factor: 6.986

10.  Deciphering Metabolic Heterogeneity by Single-Cell Analysis.

Authors:  Tom M J Evers; Mazène Hochane; Sander J Tans; Ron M A Heeren; Stefan Semrau; Peter Nemes; Alireza Mashaghi
Journal:  Anal Chem       Date:  2019-10-08       Impact factor: 6.986
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