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Literature DB >> 2414327

Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

Abstract

An indirect alkaline phosphatase immunoenzymatic staining method was developed to localise antigens in human embryo fibroblasts that have been induced by cytomegalovirus. The enzyme label was developed with a naphthol salt and fast blue to obtain a bright blue staining of the antigens that could be clearly visualised under an ordinary light microscope. The procedure is rapid, sensitive, and specific and can be used in diagnostic laboratories to detect active infection caused by cytomegalovirus.

Entities: Chemical Disease Species

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Year: 1985 PMID： 2414327 PMCID： PMC499459 DOI： 10.1136/jcp.38.10.1155

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

11 in total

1. THE IMMUNOFLUORESCENT FOCUS TECHNIQUE IN STUDYING THE REPLICATION OF CYTOMEGALOVIRUS.

Authors: F RAPP; L E RASMUSSEN; M BENYESH-MELNICK
Journal: J Immunol Date: 1963-11 Impact factor: 5.422

2. The immunoperoxidase technique for rapid human cytomegalovirus identification.

Authors: G Gerna; A Vasquez; C J McCloud; R W Chambers
Journal: Arch Virol Date: 1976 Impact factor: 2.574

3. Immediate early antigens in human cytomegalovirus infected cells.

Authors: S Michelson-Fiske; F Horodniceanu; J C Guillon
Journal: Nature Date: 1977-12-15 Impact factor: 49.962

4. Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

Authors: A Pitts; E E Williams
Journal: J Histochem Cytochem Date: 1974-01 Impact factor: 2.479

5. Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.

Authors: J G Streefkerk
Journal: J Histochem Cytochem Date: 1972-10 Impact factor: 2.479

6. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

Authors: S Avrameas
Journal: Immunochemistry Date: 1969-01

7. Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA.

Authors: M Musiani; M Zerbini
Journal: Arch Virol Date: 1983 Impact factor: 2.574

8. Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

Authors: M Musiani; C Carpi; M Zerbini
Journal: J Clin Pathol Date: 1984-02 Impact factor: 3.411

9. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection.

Authors: T H The; G Klein; M M Langenhuysen
Journal: Clin Exp Immunol Date: 1974-01 Impact factor: 4.330

10. Enumeration in whole peripheral blood of lymphocytes bearing receptors for Fc(gamma) and C3b using alkaline phosphatase-labelled reagents.

Authors: E O Pepys; M B Pepys
Journal: J Immunol Methods Date: 1980 Impact factor: 2.303

6 in total

Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

1. THE IMMUNOFLUORESCENT FOCUS TECHNIQUE IN STUDYING THE REPLICATION OF CYTOMEGALOVIRUS.

2. The immunoperoxidase technique for rapid human cytomegalovirus identification.

3. Immediate early antigens in human cytomegalovirus infected cells.

4. Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

5. Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.

6. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

7. Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA.

8. Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

9. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection.

10. Enumeration in whole peripheral blood of lymphocytes bearing receptors for Fc(gamma) and C3b using alkaline phosphatase-labelled reagents.

1. Double immunoenzymatic staining for the simultaneous detection of Epstein-Barr virus induced antigens.

2. Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay.

3. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay.

4. Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

5. Comparison of different techniques for detection of cytomegalovirus infection following bone marrow transplantation.

6. Impairment of cytomegalovirus and host balance in elderly subjects.