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Literature DB >> 2172299

Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay.

M Musiani¹, M Zerbini, G Gentilomi, G Gallinella, S Venturoli, D Gibellini, M La Placa.

Abstract

A dot blot hybridization immunoenzymatic assay for the rapid detection of cytomegalovirus DNA in urine samples was developed by using a digoxigenin-labeled probe which was immunoenzymatically visualized by antidigoxigenin Fab fragments labeled with alkaline phosphatase. A total of 516 urine samples from different groups of subjects were analyzed, and the hybridization assay was able to yield results within 24 h. The results obtained were compared with results for detection of cytomegalovirus antigens in infected cell cultures.

Entities: Chemical Gene

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Year: 1990 PMID： 2172299 PMCID： PMC268111 DOI： 10.1128/jcm.28.9.2101-2103.1990

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

Authors: M Musiani; M Zerbini; M La Placa
Journal: J Clin Pathol Date: 1985-10 Impact factor: 3.411

2. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay.

Authors: M Musiani; G Gentilomi; M Zerbini; D Gibellini; G Gallinella; S Pileri; P Baglioni; M La Placa
Journal: Histochemistry Date: 1990

3. Detection of B19 parvovirus infections by a dot-blot hybridization assay using a digoxigenin-labelled probe.

Authors: A Azzi; K Zakrzewska; G Gentilomi; M Musiani; M Zerbini
Journal: J Virol Methods Date: 1990-02 Impact factor: 2.014

4. A hybrido-immunocytochemical assay for the in situ detection of cytomegalovirus DNA using digoxigenin-labeled probes.

Authors: G Gentilomi; M Musiani; M Zerbini; G Gallinella; D Gibellini; M La Placa
Journal: J Immunol Methods Date: 1989-12-20 Impact factor: 2.303

5. Cloning of the human cytomegalovirus genome as endonuclease XbaI fragments.

Authors: D R Thomsen; M F Stinski
Journal: Gene Date: 1981-12 Impact factor: 3.688

6. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

7. A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection.

Authors: D Przepiorka; D Myerson
Journal: J Histochem Cytochem Date: 1986-12 Impact factor: 2.479

8. Detection of human cytomegalovirus in clinical specimens by DNA-DNA hybridization.

Authors: S A Spector; J A Rua; D H Spector; R McMillan
Journal: J Infect Dis Date: 1984-07 Impact factor: 5.226

9. In situ hybridization for cytomegalovirus DNA in AIDS patients.

Authors: W C Keh; M A Gerber
Journal: Am J Pathol Date: 1988-06 Impact factor: 4.307

10. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors: D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal: Virology Date: 1983-04-15 Impact factor: 3.616

3 in total