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Literature DB >> 2420755

Double immunoenzymatic staining for the simultaneous detection of Epstein-Barr virus induced antigens.

M Musiani, M Zerbini, M Plazzi, M La Placa.

Abstract

A double indirect immunoenzymatic staining was developed for the simultaneous visualization of Epstein-Barr virus-induced early antigens and virus capsid antigens in P3HR1 lymphoblastoid cell line. The double immunocytochemical staining was performed with a four-stage and a two-stage procedure employing human sera and monoclonal antibodies against Epstein-Barr virus-induced antigens, followed by the addition of specific alkaline phosphatase and peroxidase labeled antisera. The selection of substrates yielding reaction products of contrasting colours enabled the observer to distinguish cells expressing Epstein-Barr virus capsid antigens (blue) from cells expressing Epstein-Barr virus early antigens (brown).

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Year: 1986 PMID： 2420755 DOI： 10.1007/bf00493414

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

18 in total

5. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.

Authors: B M Reedman; G Klein
Journal: Int J Cancer Date: 1973-05 Impact factor: 7.396

6. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.

Authors: P K Nakane
Journal: J Histochem Cytochem Date: 1968-09 Impact factor: 2.479

7. Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

Authors: M Musiani; C Carpi; M Zerbini
Journal: J Clin Pathol Date: 1984-02 Impact factor: 3.411

1. Simultaneous immunohistochemical demonstration of antigen expression and 5-bromodeoxyuridine incorporation in plastic embedded sections.

Authors: G Harms; H van Goor; J Koudstaal; L de Ley; M J Hardonk
Journal: Histochemistry Date: 1987

1 in total

Double immunoenzymatic staining for the simultaneous detection of Epstein-Barr virus induced antigens.

1. The immunoperoxidase technique for rapid human cytomegalovirus identification.

2. Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

3. Demonstration of two distinct components in the early antigen complex of Epstein-Barr virus-infected cells.

4. Immunofluorescence in cells derived from Burkitt's lymphoma.

5. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.

6. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.

7. Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

8. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

9. Effect of heat shock on Epstein-Barr virus and cytomegalovirus expression.

10. The immunocytochemical detection of herpes simplex virus in cervical smears--a valuable technique for routine use.

1. Simultaneous immunohistochemical demonstration of antigen expression and 5-bromodeoxyuridine incorporation in plastic embedded sections.